| Objective:To investigate the protective effect of recombinant human growth hormone(rhGH) on sepsis in rats.Methods:seventy-five SD rars were randomly divided into 3 groups, sham operation group(group A), sepsis group(group B) and rhGH group(group C). Cecal ligation and puncture operation were done in sepsis and rhGH group. In addition, rhGH(1u/kg) was injected subcutaneously at the same time point operation performed in group C. Death rate of each group at 24 hours after operation was caculated and survived rats were killed in order to get blood and liver samples. Activity of ALT was tested and level of TNF-a was detected by ELISA. Bacteria translocation were detected by blood culture.Results:The survival rate in 24 hours was 100% in group A,48% in group B and 76% in group C. The activity of ALT, lever of TNF-a and positive rate of blood culture in group B and C were significantly higher than that in group A(P<0.05). The activity of ALT, lever of TNF-a and positive rate of blood culture in group C were significantly lower than that in group B(P<0.05).Conclusion:The Cecal ligation and puncture operation induced sepsis in rats were established successfully. Recombinant human growth hormone had an obviously protective effect on Cecal ligation and puncture operation induced sepsis rats. Objective:To investigate the mechanism of recombinant human growth hormone on sepsis in rats liver.Methods:seventy-five SD rars were randomly divided into 3 groups, sham operation group(group A), sepsis group(group B) and rhGH group(group C). Cecal ligation and puncture operation were done in sepsis and rhGH group. In addition, rhGH(1u/kg) was injected subcutaneously at the same time point operation performed in group C. Survived rats were killed in order to get liver samples at 24 hours after operation. The expression of TNF-a mRNA and HMGB1 mRNA were tested by real-time PCR. The expression of HMGB1 and active Caspase-3 were detected by western blot. Nucleal positive rate of NF-KBp65 protein in liver tissue was determined by immunohistochemical staining. Apoptosis of liver cell was measured by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL).Results:The expression of TNF-a mRNA, HMGB1 and active Caspase-3 in group B and C were significantly higher than that in group A(P<0.05). Nucleal positive rate of NF-κBp65 protein in liver tissue and apoptosis of liver cell in group B and C were markedly higher than that in group A(P<0.05). The expression of TNF-a mRNA, HMGB1 and active Caspase-3 in group C were greatly lower than that in group B(P<0.05). Nucleal positive rate of NF-KBp65 protein in liver tissue and apoptosis of liver cell in group C were prominently lower than that in group B(P<0.05).Conclusion:Recombinant human growth hormone can protect the liver by decreasing the apoptosis of liver cell in sepsis, inhibiting the production and release of inflammatory factors such as TNF-αand HMGB1, inhibiting activation of NF-κB in liver.
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