The Study Of Differences On T-cell Subtype Between Allergic And Non-allergic Nasal Polyps

Objectives:To quantificate the expression of T-cell related cytokines in nasal polyps and explore their roles in the development of allergic and non-allergic nasal polyps.Methods:Ten allergic and fifteen non-allergic patients with nasal polyps were studied. The T-cell subtype cytokines (IL-4, interferon [IFN]γ, IL-10, IL-17, and transforming growth factor [TGF]β) were analyzed by immunofluorescence. The levels of cytokine proteins were measured by Western Blot.Results:IFN-γ, IL-4, IL-10 and TGF-βwere expressed apparently in submucosa of all patients, but IL-17 was not easily observed in non-allergic ones by immunofluorescence. Increased levels of IFN-γ, IL-4 and TGF-βwere found in both allergic and non-allergic nasal polyps compared with control tissues (P< 0.05) However, there was no significances between them (P>0.05). Furthermore, the levels of IL-17 and IL-10 have no differences among contol tissues, allergic and non-allergic nasal polyps (P>0.05)Conclusions:Increased levels of IFN-γ, IL-4 and TGF-βwere found in both allergic and non-allergic nasal polyps, which suggested IFN-γ, IL-4 and TGF-βmay play important roles in the inflammation of nasal polyps. There was no differences of T-cell cytokines between allergic and non-allergic nasal polyps. Objectives:To quantificate the gene expression of T-cell Subtype characterized transcription factors in nasal polyps and explore their roles in the development of allergic and non-allergic nasal polyps.Methods:Ten allergic and twenty non-allergic patients with nasal polyps were studied. Expression of T-bet, GATA-3, Foxp3, and RORγt mRNA was detected by real-time polymerase chain reaction.Results:Expression of T-bet and GATA-3 mRNA was upregulated in non-allergic nasal polyps (P< 0.05). There was no significant differences on levels of T-bet and GATA-3 mRNA between allergic nasal polyps and control tissues (P>0.05). The levels of RORγt,Foxp3 mRNA have no differences among contol tissues, allergic and non-allergic nasal polyps (P>0.05). But markedly upregulated tendency of ROR Y t mRNA was showed in non-allergic ones and downregulated tendency of Foxp3 mRNA was manifested in all patients. Only the level of T-bet mRNA of non-allergic polyps was higher than allergic nasal ones (P< 0.05)Conclusions:The findings demonstrated that Th1, Th2 and Th17 cell may play a more important role in non-allergic nasal polyps. Treg cell was likely to inhibit the development of both allergic and non-allergic nasal polyps.