Expression And The Correlation Of AQP5,HIF-1α And VEGF In Human Nasal Polyps

Objective: To detect the expression of AQP5,HIF-1αand VEGF in human nasal polyps, and to observe the relationship of AQP5 with HIF-1αand VEGF, and to investigate their role in the pathogenesis of nasal polyps.Method: Using the techniques of Real-time Quantitative Polymerase chain Reaction and Western blot, the expressions of AQP5,HIF-1α,VEGF mRNA and protein were examined. The specimens were obtained from patients underwent endoscopic surgery at the same time, including eighteen nasal polyps and ten inferior turbinate tissues.Result:①The result of RT-PCR showed that the expressions of the AQP5 mRNA were lower in nasal polyps than those in inferior turbinate tissue ( P < 0.01); there was no significant difference between the expression of HIF-1αmRNA or VEGF mRNA in nasal polyps and inferior turbinate tissues (P >0.05) ;②According to the Western blot, there was no significant difference between the expression of AQP5 in nasal polyps and inferior turbinate tissues ( P > 0.05); the expression of HIF-1αor VEGF were higher in nasal polyps than those in inferior turbinate tissues ( P <0.05)③According to the Western blot, there was a positive correlation between the expression of AQP5 and that of HIF-1α(r =0.633, P < 0.05),and the correlation was also existed between AQP5 and that of VEGF(r =0.611, P < 0.05).Conclusions: AQP5,HIF-1α,VEGF are all involved in the edema formation of nasal polyps. The changes of AQP5's distribution can casue accumulation of water in nasal polyps, HIF-1α,VEGF can induce new blood vessels and increase vasopermeability in nasal polyps ; they have separate regulatory mechanism in edema formation, but might have some relationships in some ways.