The Research On Activity Of Non-alkaloid In Ephedra

Objective:Ephedra as a commonly Chinese medicine with complex chemical composition. Components have been reported alkaloids, flavonoids, yellow alkanes, tannins, volatile oils, organic acids, amino acids, polysaccharides and some phenols. At present, the ephedra has been studied broadly in worldwide, but mostly focused on the separation, identification, determination, pharmacology and toxicology in components of alkaloids and volatile oils. It uncommonly to find the research on non-alkaloid and non-volatile oils. Unfortunately, the adverse reactions about chemical composition of alkaloids in ephedra has been reported frequently at domestic and overseas. FDA has banned the use of non-prescription due to the risks of heart disease and stroke.It will bring the adverse effects to the development of ephedra. We study the components of non-alkaloids and non-volatile oils in ephedra. To research the pharmacological activity of these components on experimental hyperlipemia mice, explore the effects of mechanism and physical basis, provide some experimental evidence for development the ephedra.Methods:1 With the various means of extract, separate, purified, to get the alkaloids, polysaccharide and non-alkaloids.2 Mice were fed high fat diet to set up the model of hyperlipidemia. Mice were randomly divided into 6 groups:blank control, model, positive control, alkaloid, polysaccharide and non-alkaloid. Intragastric administration was given continuously for 4 weeks, body weight were recorded weekly. The content of TC, TG, HDL-C and activities of ALT, AST, SOD and MDA in serum were measured respectively by semi-automatic biochemical analyzer. The organ coefficient was also calculated. To research the pharmacological activity of these components in the mice of hyperlipidemia.3 To image acquisition the liver of each groups. Observe the pathological slice, to research the liver damage of mice.Results:1 Replication experimental hyperlipidemia modelMice of blank control were fed basic feed, the remaining were fed high fat diet. TC and TG in serum were increased obviously in the group of model, positive control, alkaloid, polysaccharide and non-alkaloid. Indicate that experimental hyperlipidemia model replication successful.2 The effects on mice of experimental hyperlipidemia model by non-alkaloidsCompared with the model group, the polysaccharide and non-alkaloid markedly decreased the TC, TG and increased the HDL-C in serum (P＜0.05 or P<0.01), the non-alkaloid markedly decreased the activities of ALT and AST in serum (P<0.05 or P＜0.01), polysaccharide and non-alkaloid decreased the organ coefficient of the liver, spleen and kidney in a certain degree (P<0.05 or P＜0.01). Observe the pathological slice of liver, found that the liver in group of non-alkaloid pathological changes insignificant.3 The effects on SOD and MDACompared with the bland control and model group, the non-alkaloids increased the SOD in serum significant (P<0.01), both polysaccharide and non-alkaloids decreased the MDA in serum significant (P＜0.05).Conclusion: 1 Experimental hyperlipidemia model replication successful.2 Both polysaccharide and non-alkaloids could improve dyslipidemia on experimental hyperlipidemia model with the low liver-toxicity.3 Both polysaccharide and non-alkaloids could remove freeradical in serum.