Differentially Expressed Proteins In The Interaction Between Cryptococcus Neoformans And Murine Microglia

To identify the differentially expressed proteins associated with the interaction between Cryptococcus neoformans and murine microglia. macrophage and antigen-presenting cells in the central nervous system. Methods Protein samples extracted from normal N9 cells and N9 cells co-cultured with Cryptococcus noeformans, from normal Cryptococcus noeformans strain 32609 and Cryptococcus noeformans strain 32609 phagocytosis by microglia N9 cell line. Protein samples were separated by immobilized pH gradient two-dimensional(2-D) gel eletrophoresis and identified by mass spectrum. Results The identified 15 proteins were associated with the glycometabolism, cytoskeleton, signal pathway, mental and microelement metalbolism of N9 cell., the DNA repair, Mitochondrial protein and a virulence factor of Cryptococcus neoformans. Conclusions Proteomic approaches can be used to evaluate the molecular mechanisms of cryptococcal meningitis. We also study the effect of Cryptococcus noeformans capsule depletion strain Cap64 on the apoptosis of microglia N9 cell line,suggesting that the capsule was not the only factor inducing apoptosis on microglia. Other virulence factors in Cryptococcus neoformans induce cell apoptosis and inhibit cell activity.