Studies On The Quality Control Of Crude Drugs And Therapeutic Substance Of Wei-Chang-An-Wan

The purpose of this study is to control the quality of Wei-Chang-An-Wan throughthe quality control of the crude drugs, which was chosen according to the compositionof the formulation. On this basis, changes in the chemical compositions of Rheumpalmatum and Fructus Aurantii were studied. In order to explain the rational use ofWei-Chang-An-Wan, this study was also to evaluate the anti-inflammatory andanalgesic actitivites.We developed a new method to determine the content of five anthraquinones inthe Rheum palmatum. Further more, we used this method to evaluate the drugscollected from different areas and found that the quality of the herbals collected fromGansu province was the best and the contents was2%. For Magnolia officinalis, aHPLC-UV method was developed for the determination of magnolol and honokiol.With the highest contents3.34%of magnolol and honokiol, the herbal collected fromHubei province was the best. When compared the polysaccharides of Fructus Jujubae,the herbals collected from Hebei and Shandong provinces showed the same contents.However, herbals from Hebei province were the best choice from the whole point. ForRhizoma Chuanxiong, the contents of ligustrazine and ferulic were used as theevaluation criterion. The results showed that Doujiang Weir as the genuine RhizomaChuanxiong producing area produced the best herbs.We determined the contents changes of five anthraquinones from Rheumpalmatum and four flavonoid glycosides from Fructus Aurantii at the compatibility ofRheum palmatum and Fructus Aurantii. The anthraquinones from2.39%to3.30%while flavonoid glycosides could increase from7.55%to9.38%. The results showedthat all the contents of them were increased.Four inflammation models including three acute inflammation models of earedema induced by xylene in mice, paw edema induced by carrageenan in rat andincreased capillary permeability induced by acetic acid in mice and one chronicinflammation model of granulomatous tissue induced by cotton pellet implantation inrat were used to evaluated the anti-inflammatory activity of Wei-Chang-An-Wan. Theresults showed that it could inhibit all of these inflammation models with theinhibition over30%. We used acetic acid-induced abdominal writhings, formalin andhot plate test to research the analgesic activities of this formulation. Wei-Chang-An-Wan could reduce the number of writhings with the inhibition rate84.69%, and decreases the licking time in the formalin test with the inhibition rate40%, but not hot plate test.Phenol red was used as a marker to investigate the effects ofWei-Chang-An-Wan on motility of small intestine and gastric emptying in mice. Theresults suggested that Wei-Chang-An-Wan possessed spasmogenic and spasmolyticproperties, and the active parts might be P.E. extracts of the Methanol extracts ofWei-Chang-An-Wan.In conclusion, all these researches provide a theoretical support for the herbalsquality control, lay the foundation for the anti-inflammatory and analgesic activities,and supply the basis for the clinical application and further development ofWei-Chang-An-Wan.