Effects Of Ration Of Different Seasons On Microbial Flora And Environment Of Rumen And Biochemical Indicators Of Serum For Reo Deer

Reo deer is a kind of ruminants with the high economic value. In the wild, theanimal exists on a diet of plant feed, such as forage grass, nuts and fruits. Under theartificial rearing, the ration bases on plant-based feedstuffs with little concentrated feeds.Different feed source and nutrient levels will have effects on the species and quantitesof rumen microbe. In this experiment, we study the effect of different ration in summerand winter seasons on microbial flora and environment of rumen and biochemicalindicators of serum by terminal restriction fragment length polymorphism (T-RFLP)and fluorescent quantitation PCR. This lays on a foundation for optimization of rationstructure and satiation of nutritional needs of reo deer.In the present experiment, the effect of different ration in summery and winterseasons on ruminal microflora in ruminal content of roe deer was observed. Theresults showed that, with the concentrate-to-forage increasing, the content ofStreptococcus bovis，Lactobacillus ruminis，Ruminibacter amylophilus，Megasphaeraelsdenii and Selenomanas ruminantium in summer was higher than those of winter(P<0.05),while the contents of Fibrobacter succinogenes，Batyrivibrio fibrisolvens，Ruminococcus albus and Ruminococcus flavefaciens was lower than those of winter(P<0.05). The proportion of rumen methanogens was decreased (P<0.05). There wasobvious change in content of rumen ciliates, the proportion of Diplodinium andEpidinium was decerased, and the proportion of Entodinium and Ophryoscolex wassignificantly increased in summery (P<0.05).We evaluated The effect of different ration in summery and winter seasons on PHand the concentration of VFA, NH3-N, BCP, endotoxin and histamine in ruminalcontent of roe deer. The results showed that, with season altering, the content of crudeprotein in ration was increased; the content of NH3-N and BCP in summer was higherthan those of winter(P<0.05), the PH of rumen content and the concentration ofacetate were decreased(P<0.05), and the concentration of propionate was increased insummer(P<0.05), while the variational level of endotoxin and histamine in rumen wasinsignificant(P>0.05). It suggested that, the two kinds of ration could supply a nutritional requirements for roe deer and maintain the equilibrium of rumenenvironment, and the ration of summer is more suitable to roe deer.We also surveyed the effect of the ration in summer and winter on biochemicalindicators of serum. The results showed that the activities of ALT, AST, and theconcentrations of GUL, GLU, TC, TG, Ca and Zn in serum of roe deers were higher insummery ration than those of winter ration(P<0.05), the concentrations of BUN,NEFA, CREA and UA were decreased significantly in serum of roe deers fed onsummery ration(P<0.05). It suggested that the different kinds of rations could affectthe biochemical indicators in serum of roe deers, and the ration of summer is moresuitable to nutritional requirement for roe deers.