Regulation Of Microecologic Preparation On Ruminal Microbial Flora In Artificial Induced SARA Goat

In recent years，with the rapid development of the rearing industry in China,ruminant farming has initially carried out a large-scale, intensive industry model.Subacute ruminal acidosis （SARA） is a common disease in fattening beef, goat anddairy cow with the high intensive model, its incidence rate ranges from19%to26%.Annual economic losses have been5million to10billion dollars in America. Theprimary cause of Subacute ruminal acidosis is the pursuit of rapid fattening, thenfeeding a lot of concentrated feeds，meanwhile reducing the intake of roughage in theproduction process. So it results in the accumulation of the rumen organic acid,especially VFAs, and the dysbacteria. The rumen epithelial cell structure and barrierfunction were destroyed by high concentration of VFAs in the rumen， and theruminal epithelium horny insufficiency and mucosal injury were caused subsequently.SARA can trigger rumen inflammatory reaction （such as rumen ulcer and rumeninflammation）, and cause systemic inflammatory disease （such as laminitis and liverabscesses）. It is unclear about the variation of microbial flora in rumen caused bySARA and regulation of microecologic preparation on ruminal microbial flora atpresent.Six goats with30³5kg BW were selected to establish the SARA model bygradually increasing the ratio of concentrated feeds to forage. Using the terminalrestriction fragment length polymorphism （T-RFLP） technology and quantitative PCRtechnology analysed the rumen microbial flora of different diet stages and rumen pH.Goats with induced SARA were administrated by probiotics combited with yeast andruminal bacteria to evaluate regulation of the rumen microbial flora.The experiment showed that the concentration of VFAs in the rumen and bloodsignificantly increased in induced SARA goat. The proportion of acetate wasdeclined,and the proportion of propionate and butyrate was increased in the VFAscomponent. The fermentation type was changed from acetic acid-type to propionicacid-type. The concentration of LPS and histamine was increased in rumen and blood,which causes the body’s inflammatory response. The number of lactate-producing bacteria,lactate-utilizing bacteria and amylolytic bacteria was significantly increased，and the number of cellulose decomposition bacteria was declined. The concentrationof pCO₂and Na⁺in the blood was increased, and the HCO₃^-and Ca²⁺was decreased,which showed acidosis and electrolyte imbalance. The concentration of ALT and ASTwas increased, indicating the abnormal liver function. The concentration of CREA,BUN, GLU, TG, TC and TP was increased, which showed the deprivation of bodyfluids.Compound probiotics was administrated to induced SARA goats, which iscomposed of Saccharomyces cerevisiae, Burton Pichia yeast, Candida utilis,selenomonas ruminantium and megasphaera elsdenii. The number of selenomonasruminantium and megasphaera elsdenii was significantly increased in the rumen aferfeeding probiotics. The number of other bacteria was increased except lactobacilli andPrevotella. The concentration of VFAs in rumen and blood was decreased, and theruminal pH was increased. The concentration of LPS and histamine was decreased inrumen fluid and blood. The pCO₂was decreased, and the concentration of HCO₃^-wasincreased. Then the symptoms of acidosis were alleviated. The concentration of ALT,AST and CREA was decreased, but the concentration of BUN, GLU, TG, TC and TPstill maintained a high level. In conclusion, the compound probiotics is useful for therecovery of ruminal dysbacteria and the alleviation of symptoms caused by SARA.