Research On Agricutural Activities And Biomimetic Synthesis Of Murraya Paniculata

Murraya paniculata L. is belong to Murraya species of Rutaceae genus. Murraya paniculata have important pharmacological effects, such as anti-fertility, anti-inflammatory, lowering blood sugar, anti-tumor effects; Murraya paniculata have important agricultural activities too, such as antifeedant activity, fumigation activity, antifungal activity. Based on the early research about insecticidal activity of Murraya paniculata, this paper will further search the type and structure of the active substance, and will design and synthesize analogues of metabolites from Murraya paniculata, the results are as follows:1, Research progress has been reviewed about Murraya paniculata in domestic and foreign, especially metabolites structures of Murraya paniculata, which will help to design and synthesize analogues.2, Using active tracking methods, chloroform extract of Murraya paniculata showes significant contact activities against the test insects. So, the component of chloroform extracts are isolated by chromatography; Among L3component exhibits most excellent activities;3,5,6,7,3’,4’,5’-heptamethoxyflavone and3,5,7,8,3’,4’,5’-heptamethoxyflavone of L3component are further purified and separated by chromatography; LC50values of3,5,6,7,3’,4’,5’-heptamethoxyflavone are0.1147mg/mL and0.1201mg/mL against Musca domestica and Lipaphis erysimi. Which are lower than the LC50value (0.3243mg/mL) of0.3%azadirachtin SP.3, Flavonoids of Murraya paniculata are the first innovative utilization. Total flavonoids of Murraya paniculata leaves and stem are extracted using macroporous resin by optimizing industrialization method, and the flavonoids are formulated into a soluble powder. Insecticidal activity showes that flavonoids have significant contact activity against the test insects, and the effect is the same as the azadirachtin. The flavonoids can effectively control Lipaphis erysimi.4, At the first time, the mode of action of flavonoids are proved to inhibit acetylcholinesterase, carboxylesterase and glutathione-S-transferase of Musca domestica and Lipaphis erysimi.3,5,6,7,3’,4’,5’-heptamethoxyflavone can inhibit acetylcholinesterase and carboxylesterase of Musca domestica, so the acetylcholines are accumulated in housefly body, carboxylesterase weaken detoxification, which lead to Musca domestica poisoning; Flavonoids also inhibits acetylcholinesterase and glutathione-S-transferase of Lipaphis erysimi, which make acetylcholine accumulation, glutathione-S-transferase detoxification weaken, Lipaphis erysimi poisons to die. 5, Total flavonoids of Murraya paniculata exhibits a strong antioxidant effect. Reduction, inhibition of lipid peroxidation, hydroxyl radical scavenging effect are significantly better than the positive control, vitamin E.6, At the first time,2-aryl-6H-furo[2,3-g]coumarin44compounds and2-substituted amides-3-carboxylate-4-aryl-7,7-dimethyl-tetrahydrochromeno20compounds are desigined and synthesized according to analogues of Murraya paniculata.Insecticidal activity showes that:the title compounds I dd, I hh, I oo have significant photoactivated insecticidal activity against Aedes albopictus at the concentration of100mg/L, especially compound I oo activity, which is the same activity as the control a-triple thiophene; also found compounds I hh and I oo show direct contact activities at high concentrations.Fungicidal activity showes that:the compounds I i, I hh, I kk, I oo, I uu manifest fungicidal activity over80%against Colletotrichum gloeosporioides, Fusarium oxysporum, Pestalotia palmarum at150mg/L, which is the same fungicidal activity as the chlorothalonil. Inhibition rates of compound II a are61.6%and66.7%against Colletotrichum gloeosporioides and Colletotrichum musae respectively, which is th e same inhibition activity as the chlorothalonil.Structure activity relationships showes that:the substitute are multifluoro phenyl group or a thienyl group at2-furocoumarin, the compounds have significant photoactivated insecticidal activities.