Based On The Technology Of Dynamic Phosphorus Magnetic Resonance Spectrum Of Skeletal Muscle Mitochondrial Function In Type 2 Diabetes Lower In The Body

Purpose: To evaluate the skeletal muscle mitochondrial function using in vivodynamic phosphorus magnetic resonance spectroscopy(31P-MRS) on clinical3T MRsystem.Materials and Methods:31P-MRS was acquired from skeletal muscles in47healthyvolunteers, spectra were analyzed using matlab software. concentration of inorganicphosphate(Pi), phosphocreatine(PCr) and adenosine triphosphate(ATP) of every statewas measured from area under the peak separately. Concentration of adenosinediphosphate(ADP) and intracellular pH value was calculated from the biochemistryreaction equilibrium.Results: During exercise, PCr concentration decreased, Pi, Pi/PCr and ADP valuesincreased, in recovery state, Concentration of every phosphate compound come backto the resting state gradually.Conclusions:3T high field intensity dynamic phosphorus magnetic resonancespectroscopy technique can reflect skeletal muscle mitochondrial function in vivo andprovide valuable information to evaluate muscle functional imaging, Lay a foundationof researching muscles related diseases. Purpose: To evaluate type2diabetes patients and impaired glucose tolerance persons’skeletal muscle mitochondria function using in vivo dynamic Phosphorous MagneticResonance Spectroscope (31P-MRS) on3T MR scanner. Moreover, to assess therelationship between skeletal muscle mitochondria function and blood glucose insulinlevels, insulin resistance index.Materials and Methods: Subjects included18DM2patients、21impaired glucosetolerance (IGT) persons and20volunteers with normal glucose tolerance (NGT).Three groups were matched for age, gender, and body mass index (BMI).Noninvasive31P-MRS was acquired from quadriceps muscles of each subject duringrest, exercise, and recovery periods. The concentration of Pi, PCr, and β-ATP weredetermined from peak area using Matlab program. The value of ADP, PH, and thePi/PCr were calculated. The initial recovery rate for PCr and ADP were generatedfrom the mono-exponential fitted recovery curve. Fasting blood glucose content,blood glucose content2hours after meal were measured in three groups for relatedendocrine experiments, fasting insulin levels and insulin resistance index weremeasured in DM2group and IGT group.Results: PCr and ATP content in DM2group and IGT group in the resting state werebelowless than those in normal control group (P <0.05).PCr, the Pi, β-ATP content inDM2and IGT group when the motion stops was lower than those in control group (P<0.05). PCr recovery rate in DM2and IGT group during recovery stage was significantly lower than those in the normal group. And PCr recovery rate in DM2group was lower than that in IGT group (P <0.05). Fasting blood glucose content andblood glucose content2h after a meal in DM2and IGT group were significantlyincreased compared with normal group (P <0.05). Fasting insulin content in IGTgroup was significantly higher than that in DM2group (P <0.05), and insulinresistance index in DM2group was significantly higher than that in IGT group.Conclusions: Skeletal muscle energy state decreases, and skeletal musclemitochondria oxidative phosphorylation function is damaged in patients with DM2orIGT. The patients with DM2have more serious impairment of skeletal musclemitochondria oxidative phosphorylation function and higher insulin resistance level inpatients compared with IGT persons.