Study On The Key Technologies Of Diosgenin Clean Production And Resources Utilization Of Dioscorea Zingiberensis

Diosgenin is the basic raw material of synthesising ofcorticosteroids, the adrenal hormones, protein anabolic hormones,which is known as "the second discovery of the20thcentury inpharmaceutical industry". Rothrok’s method process was simple, lessinvestment in equipment, low cost, which drew enterprises’s attention ofall ages. In recent years, however, with the worsening of theenvironment, the pollution problems of Rothrok method cause a greatconcern and the process was being challenged. In this paper, withDioscorea Zingiberensis as research object, for the purpose of reducingpollution and cost, key technologies of diosgenin clean production andresources utilization of Dioscorea Zingiberensis were studied, which were described as below:1All kinds of methods were applied to determine the content ofstarch, cellulose, hemicellulose, lignin, diosgenin in DioscoreaZingiberensis, and laid the foundations for Dioscorea Zingiberensisresources utilization.2With Dioscorea Zingiberensis as the raw material, ethanol asextraction agent, the yield of dioscin and diosgenin as the evaluationindexes, the single factor and the orthogonal experiment methods wereused to optimize the conditions of ultrasonic extraction of dioscin.When ethanol volume fraction was75v/v, the ration of solid (DioscoreaZingiberensis) to liquid (extractant) was1:5, the ultrasonic frequencywas35.74KHz, ultrasonic time was30min, Dioscorea Zingiberensiswas continuously extracted for3times, the yield of the dioscin anddiosgenin can reach to14.49%and3.2907%, which were1.36times,1.13times of the extraction at room temperature (traditional extractionmethod), respectively. The extraction efficiency was32times of the traditional extraction method.3Under certain circumstances of ultrasound conditions (frequency,time), the influence of surfactant type, structure, hydrophilic lipophilicbalance (HLB) value, the critical micelle concentration (cmc) ondioscin yield were researched. The feasibility of surfactant selectionprinciple at dioscin extration was studied according to the influence ofsurfactant on dioscin molar solubilizatoin ratio (MSR), serving as atheoretical basis for the choice of surfactant in dioscin extraction.4The investigation on the influence of flocculant types, dosage,flocculation temperature, flocculation time on the dioscin separationfactor help to determine the optimum flocculation conditions of dioscin,along with the study of the influence of the sulfuric acid dosage, theconcentration of sulfuric acid, the number of times sulfuric acid reuseand hydrolysis temperature of dioscin, hydrolysis time, diosgeninextraction time on the yield of diosgenin.5On the basis of laboratory experiment, pilot-scale experiments were conducted for5times with50Kg Dioscorea Zingiberensis as rawmaterials. A comprehensive evaluation of production costs of rawmaterials, waste water emissions and properties of waste water weremade, and considered that the process could be achieved the aim ofpollution control and costs reduction in the production of diosgenin.6Modern biotechnology is adopted to optimize the conditions ofalcoholic fermentation with Dioscorea Zingiberensis residue after theextraction of dioscin as a raw material. With30Kg above-mentionedraw material, pilot-scale experiments demonstrate that alcohol contentof the fermented mash is up to10.5v/v, starch utilization90.6%. Thistechnology makes resource utilization starch in the DioscoreaZingiberensis feasible, and further reduces the cost of production ofdiosgenin in line with the requirements of low-carbon, environmentalfriendly development.The innovation of this paper.1The technology of diosgenin clean production was research systematically, With surfactant extraction technology and flocculationtechnology as the core. On the basis of laboratory experiments,wastewater discharge and wastewater properties in the process ofdiosgenin clean production are studied. The costs of raw materials arecompared in the process of diosgenin clean production and thetraditional process. It is found that the clean process helps to controlpollution and reduce costs.2The effect of surfactant structure on dioscin extraction is studiedin several ways, which paves the way for the scientific surfactantselection principle at dioscin extraction. Besides the test of surfactantson dioscin molar solubilizatoin ratio (MSR), verified feasibility ofsurfactant selection principle at dioscin extraction with the effect ofsurfactant on molar solubilization rate.3The content of alcohol in fermenting mash, the utilization rate ofstarch and the SEM of distillers’ grains are compared with DioscoreaZingiberensis and Dioscorea Zingiberensis residue as raw materials. This technology is in line with the development of recycling economymode, laying the foundation for comprehensive utilization of otherstarch-containing plants.