| Background:Stroke is the common and frequently-occurring disease overworld which with high incidence, high morbidity and high recurrence rate. There are150-200million new cases each year in China. Ischemic stroke is the most common type of stroke, accounting for60-80%of all stroke. More and more studies show that the inflammatory process involved in stroke damage cascade, the main reason may be caused by cerebral ischemia and reperfusion injury. Early thrombolysis, anticoagulation therapy are the effective treatment recognized by evidence-based medicine, but there is strict time window, the effect was not significant in the long-term recovery process.A large number of clinical and experimental studies have shown that acupuncture is an effective treatment to ischemic stroke, and have shown significant effect in acute, recovery, sequelae and rehabilitation phase.Objectives:The subject observed the the dynamic change of stress-damage-repair signal chain in the peripheral blood seram and ipsilateral/peripheral brain tissue after acupuncture intervention on cerebral ischemia and reperfusion model rats. To explore acupuncture treatment’s precise mechanisms in cerebral ischemia and reperfusion inflammatory injury. To provide the esact laboratory evidence for clinical treatment programs.Methods:1, The rats were randomly divided into the control group, sham group and model control group,,10animals of each group. The model animal were evaluated by Neurological score, TTC staining(to observe cerebral infarction), and HE staining(to observe the morphological changes of the brain tissue).2, The rats were randomly divided into the control group (10animals), sham group (10animals) and model group, model animals were divided into model control group, nimodipine group, acupuncture group(Baihui, Yintang and Renzhong) according to the random number table, each group was divided into6schedule groups-12h,24h,48h,72h,96h,144h,10animals of each schedule. Chose ELISA method to detect the inflammatory response related signals in serum, including stress signal-ACTH, endogenous danger signal-HSP70, start-up and adjustment signals-IL-6, IL-1β, signal of damage effect-TNFα; chose the immunohistochemical technology to detect the expression of inflammatory response signals in ipsilateral and contralateral brain tissue, including activate and adjust signals-IL-6, IL-1c, signal of damage effect-TNFα, MMP-9, contact signal-ICAM-1, MCP-1, and the repair signal-TGFp.3, The rats were randomly divided into the control group (10animals) and model group, model rats were divided into model control group, acupuncture treatment groupl (Baihui, Yintang and Renzhong), and acupuncture treatment group2(Baihui, ipsilateral Zusanli a), according to the random number table, then divided into12h,24h,48h,72h,96h,144h,6schedule in each group,10animals of each schedule. Take brain tissue, frozen sections, used the immunohistochemical technology to detect the expression of IL-6, TNF-α, MCP-1, TGFβ in contralateral brain tissue.Results:1, Model EvaluationThe TTC staining found that there was typical dyeing missing in local ischemic brain tissue, appear white, in line with the disposable area of the middle cerebral artery,and the infarction was relatively stable before and after the optic chiasm. HE staining were observed that, staining shallow in the central farction area, decrease of the cells number, nuclear condensation, smaller and irregular of the nucleolus; interstitial edema; endothelial cell swelling; a large number of grid-like vacuole.2, The influence of acupuncture treatment to inflammatory response signals in serum: The inflammatory response signals ACTH, IL-1β, IL-6, TNF-α, Hsp70, in cerebral ischemia and reperfusion model rats’ serum, showed typical bimodal curve mode in the process of damage development, and the trend were similar. ACTH reached the first peak at12h, and reached the second peak at48h; IL-1β, IL-6, TNF-α, Hsp70reached the first peak at24h, an second peak-to-peak at72-96h. ACTH, IL-6, TNF-α, Hsp70of sham-operated group showed similar bimodal expression trend, IL-1βof sham-operated group was higher than physiological levels only at72h.Electro-acupuncture treatment on Baihui, Yintang and Renzhong could significantly lowered the the first peak’expression levels of inflammatory response signals ACTH, IL-1β, IL-6, TNF-α, and Hsp70in peripheral serum; could significantly lowered the second peak’ expression levels of ACTH, IL-1β, TNF-α; could increase the second peak’ expression level of IL-6.3, The influence of acupuncture treatment to inflammatory response signals in the brain tissue:The inflammatory response signals in cerebral ischemia and reperfusion model rats’ local brain tissue, IL-6, IL-1β, MMP-9, ICAM-1, MCP-1, TGFβshowed typical bimodal curve mode in the process of damage development, and the trend were similar, reached the first peak at12-24h; IL-6reached the second peak at96h; IL-1(3, MMP-9reached the second peak at48h; ICAM-1, MCP-1, TGF(3reached the second peak at72h; TNFαcontinued to increase at12h, reached rhe first peak at24h, maintain a high level to72h. Sham operation group showed similar expression trends.The inflammatory response signals in cerebral ischemia and reperfusion model rats" contralateral brain tissue, IL-6, IL-1β, TNFα, ICAM-1, MCP-1, TGFp showed typical bimodal curve mode in the process of damage development, to reached the first peak at12-24h; IL-6reached the second peak at96h; IL-1βcontinued to increase from48h, reached the highest value atl44h; TNFareached the first peak at48h; ICAM-1, MCP-1, TGFβreached the second peak at72h; MMP-9reached the first peak at48h, continued to increase from72h, reached the highest value at144h. Sham operation group showed similar expression trends. Electro-acupuncture treatment on Baihui, Yintang and Renzhong had significantly influences on inflammatory response signals in bilateral brain tissue. For the ipsilatcral brain tissue, the expression levels of IL-6, IL-1β, TNFα, MMP-9, MCP-1, TGFβ were lowered; for the contralateral brain tissue, the expression levels of IL-6, MMP-9, MCP-1. TGFβwere lowered; in contralateral brain tissue, the expression levels of IL-6, MMP-9were raises in the acute damage phase at48h; the expression of ICAM-1showed a rising trend before72h in the ipsilateral brain tissue, and before48h in the contralateral brain tissue.After acupuncture treatment, the second peak’ expression of IL-6, ICAM-1in Ipsilateral brain tissue were in early appearance; the second peak’ expression of IL-6, IL-1β, MMP-9, ICAM-1, MCP-1TGFβ in contralateral brain tissue were in early appearance.4, The influence of different acupoints to inflammatory response signals in the local brain tissue: Electro-acupuncture treatment on Baihui, Zusanli, contrast the points Baihui, Yintang and Renzhong, could significantly lower the inflammatory response signals’expression levels in the ipsilateral brain tissue in the rat model, including IL-6, TNF-α, MCP-1, TGFβ, could show the second peak of IL-6, TGFβ in advance.Conclusions:1, The brain ischemia reperfusion rat model was stable; cerebral ischemia-reperfusion injury activated the inflammatory/immune-related stress-damage-repair signal chain in body part and the whole, including peripheral serum, bilateral brain tissue; this model was copied successfully.2, Electro-acupuncture treatment on Baihui, Yintang and Renzhong played the network regulation on stress-damage-repair signal chain, had the overall impact to the inflammatory response signals in he peripheral serum and bilateral brain tissue, including stress signal, endogenous danger signal, start and regulate signals, damage effect signals contact signals, and repair signal; electro-acupuncture may reduce ischemia-reperfusion injury through inhibiting excessive stress, reducing the inflammatory injury, promoting inter-cell contact, starting the repair function in advance, adjusting the healthside brain tissue to compensatory.3, Electro-acupuncture treatment on Baihui, Zusanli, contrast the points Baihui, Yintang and Renzhong, had better influences in regulating the body’s inflammatory stress response to injury, reducing the inflammatory response and inflammatory, activating repair function; combined use of acupoints on the head and body may be better in reducing inflammatory injury than using the head acupuncture points only. |
PDF Full Text Request