| Object iveChronic atrophic gastritis (CAG) is the common disease in the digestive system, the disease often is persistent and difficult for cure. The most serious consequences it may cause is precancerous stomach cancer or stomach cancer. As the development of medicine, more and more attention are being paid by the medical specialist circle in carrying out various research perspectives on diagnosis and treatment.CAG is a type of chronic gastritis, with the major pathological sign is that the gastric excretory gland is atrophied, with loss of gastric glandular cells and corrosive gastric atrophic mucosa, the muscular layer is thickened. Chronic gastric mucosal atrophy is viewed by the traditional medicine with symptoms known as:"Epigastric pain/Stomachache","Epigastric Fullness""hiccough","Belch","Vomit "... The main cause is due to disorders the functions of the spleen and stomach, pathogenic mechanism is mainly due to weakened spleen-stomach, Blood depression&Qi stagnation, moist heat Within the depression. Based on dialectical methodology combined with clinical experience, the remedy Weipixiao was researched and developed which has the effect of strengthening the stomach and blood, clearing the blood stagnation, clear the heat, detoxification (including the medical herbs Taizishen (Radix Pseudostellariae Heterophyllae), Huangqi (Astragalus membranaceus); Baizhu (Atractylodes macrocephala)Danshen (Salvia miltiorrhiza) Baihuasheshecao (Hedyotis diffusa).... In this design, we caused the model of rat with Chronic gastric mucosal atrophy and administered with Weipixiao for treatment to study on the mechanism of recovering effect and gastric mucosa protection.Purpose:To study the mechanisms of gastric mucosal protection through a number of criteria:the gastric acidity, the change of the micro blood-vessel of the gastric mucosa, levels of SOD, MDA serum and evaluation recovery of gastric lining of the rat with gastric atrophy through the ultramicroscopic structure image. Via this, the initial evaluation on mechanism of the effect of Weipixiao will be made to serve as a scientific basis for the treatment of gastric mucosal atrophy in clinically.MethodsSelected80Wistar rats, weighing200±20g.10of them was used as the control group,70was put in a model for causing them diseased. The control group was fed in normal condition, the diseased model were mainly injured by the method of using chemical. After8weeks of causing disease, killed10of them to make dyed stomach specimens to ascertain the successful creation of a diseased pattern, then the rest of60rates was divided into5groups x12rats:comparison pattern, vitacoenzym, high-dose weipixiao, medium-dose weipixiao and low-dose weipixiao. Giving the rats continuous medication for8weeks, at the end of the16th week, killed all rats, measured the amount of gastric acidity, observed the mucosal structural changes, gastric ultramicroscopic blood image of gastric mucosa, quantified the SOD activity, content of MDA serum.ResultsRats were successfully caused with CAG:disease-caused rat model had excretory glands shrunken, reducing in the quantity; gastric mucosa was thinly atrophied, muscular layer of mucosa was thickened. It is possible to observe that the inflammatory dense cell infiltration, even including formed cluster of lymphocytes. Moreover, it is possible to see heteromorphy, pyknomorphous thymocyte, the karyoplast also changed not according to the rule, atypical proliferative image from moderate to severe degree.The groups of rats given with high, medium and low doses of Weipixiao all show change with thickened gastric mucosa, with more proliferative glands, with numerous new images with small size. It is seen in some areas the image of glands in chaotic order, and images of chronic inflammatory infiltration cell. Namely,100%in diseased rat group had sign of chronic infection with manifestations ranging from mild, moderate to severe. The majority is severe cases, compared with control group, the difference of statistical significance with P<0.01. In vitacoenzyme-used rat group50%of the rats still had chronic inflammation compared with diseased rat with differences. For3groups of rats which was given weipixiao, only had inflammatory images was note with25%to33.33%compared with the diseased rat group, the difference is statistically significant with P<0.01.In the diseased rat model groups,11rats had signs of mucosal atrophy from mild to severe degree, accounting for91.67%, compared with the control group. The difference is statistically significant with P<0.01. In the vitacoenzyme Group, still7rats with signs of mucosal atrophy was noted, accounting for58.33%, the rate is reduced compared with the diseased model group but the difference is not statistically significant. The3groups are obsersed to have glandular regeneration, only remain with atropic image33.33%to41.67%, compared with the model group, showing difference with P<0.05.The diseased group75%show intestinal tract or atypical hyperplasia, the group administered with vitacoenzyme and weipixiao have lower rates, but the difference was of no statistical significance. The groups had no cancer imaging.The diseased group has gastric mucosa thickness showing clearly the average atrophy347.0±45.50μm compared with the control group655.3±42.06μm, the difference is of statistical significance. The Vitacoenzyme group and3groups using Weipixiao have mucosal thickness changed from448.8±47.40μm to539.8±69.40μm, compared with the diseased group difference was noted, but compared with the control group no difference was noted.The pH value of gastric juice of the diseased rat model considerably increased3.02±1.13(compared with control group was1.45±0.67), total acidity decreased112.54±7.54mmol/L (compared with the control group225.60±18.74mmol/L) expressed in glands of the atrophic gastric mucosa and reduced gastric secretion. A clear reduction of pH value of the weipixiao group compared with the diseased group was noted from1.37±0.53to1.84±0.95, the total acidity increased from202.25±7.09mmol/L to230.65±11.29mmol/L, the difference is statistically significant. This result proves that the remedy has the effect of recovering gastric mucosal cells functioning the gastric acid secretion.The density of microscopic blood vessel of gastric mucosa in model group was significantly reduced (10.25±4.58) compared with the control group (30.90±9.00) the difference is statistically significant with P<0.05. The Weipixiao, vitacoenzyme groups have sign of increased number of microscopic blood vessels close to the control group (from19.25±5.99to25.25±9.42), the statistics show that there is no difference between Weipixiao high-dosed group compared with the control group.Serum SOD activity in CAG rat of disease-caused model group was significantly reduced, the MDA levels was highly elevated, compared with control group P<0.05. The SOD activity increased significantly (P<0.05) of the vitacoenzyme and3Weipixiao groups was noted with significantly increased (P<0.05), MDA level was remarkably decreased (P<0.05). The result has proven that the Weipixiao has effect of eliminating oxidization substances via the SOD enzyme, demonstrated in the reduction of MDA level.Conelusion1. The Weipixiao remedy has effect of improving the gastric mucosa inflamation in CAG rats, restoring and regeneration of the atrophic glands. This means that mucosal thickness is increased, partly improving the phenomenon of atypical proliferative and appearance of intestinal gland.2. With the effect of reducing the pH value, increasing gastric acidity, this demonstrates that Weipixiao has effect of restoring the gastric mucosa cell, regulating the balance of reproduction and destruction by gastric epithelium cells.3. The clearing effect to the blood stasis of Weipixiao in restoring the density of micro blood vessels which is reduced because of chronic inflammatory atrophy by promoting to blood supply of the atrophic mucosa.4. The remedy has protective effect to the gastric mucosa, preventing cell damage by increasing the activity of SOD, reduction of MDA serum content.The remedy "Weipixiao" has good effect for treatment of rats with chronic gastric mucosal atrophy in experiment, helping the recovery of atrophic mucosa. The mechanism may be associated with the restoration of density of micro blood vessels, recovery of mucosal cells, strengthening the system to prevent cell damage, antioxidant, controlling the gastric acidity secretion. |
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