Massage For Sciatic Nerve Injury Rat Model Ngf And Its Receptor Selection Research

ObjectiveObserve the influence of Tuina therapy to the behavioristics, morphology, NGF, TrkA and p75NTR of sciatic nerve injury model rats. Probe the biological mechanisms of Tuina therapy in sciatic nerve injury.MethodUse the nerve clamping method to make the sciatic nerve injury model. All the rats are devided into5groups with two-time randomgroup design.5groups are blank control group,-sham-operated group, model control group, sham-Tuina group and Tuina group. Behavioral indexes including PWL test, board test, weight, food consumption in24h. Morphological indexes including HE of medulla spinalis and gastrocnemius muscle, counting of medulla spinalis nucleus and diameter of gastrocnemius muscle cells. NGF, TrkA and p75NTR is measured by immunohistochemical method as result of qualitative analyses. NGF, TrkA and p75NTR is measured by western blot method as result of quantitative analyses. Immunohistochemical results are analysed by Image pro plus6.0. Western blot results are analysed by Quantity One. All the datas are analysed by SPSS.ResultThe PWL test scores of the model group are higher than the blank control group in0,10and20times of treatments (P<0.05). Tuina group’s score is much lower in10and20times compared with model group (P<0.05). The board test scores of the model group are lower than the blank control group in0,10and20times of treatments (P<0.05). Tuina group’s score is much higher in10and20times compared with model group(P<0.05). The weights of model group rats are lower than the blank control group in0,10and20times (P<0.05). Tuina group rats’ weights are higher than model group (P<0.05). Food consumption result in24h shows the same ones of weights. In counting results of medulla spinalis nucleus, Tuina group is higher than model group in10and20times (P<0.05). In diameter results of gastrocnemius muscle cells, Tuina group is lower than model group in10and20times (P<0.05). In the result of NGF and TrkA, the model group is higher than blank control group(P<0.05), tuina group is higher than model group (P<0.05). In the result of p75NTR, the model group is higher than blank control group (P<0.05), tuina group is lower than blank control group (P<0.05).Conclusion Tuina treatment is effective to improve the feeling function and motor function of the sciatic nerve injury rats. Tuina is effective to reduce the pain of rats. NGF, TrkA of medulla spinalis are improved by tuina. p75NTR of medulla spinalis is reduced by tuina. In this case, NGF and TrkA would start the nerve regenerat ion mechanism. In the other hand, NGF and p75NTR would stop the the neural apoptosis mechanism. As a result, tuina can change the NGF and its receptor. NGF would choose different receptor by tuina treatment. This could be one of the mechanisms of tuina’s effects.