| Objective: Previous studies demonstrated the antineoplasmic activity ofpulsatilla chinensis polysaccharides (PCPS) is various. In this study we discussed theinhibitory effect of PCPS on glioma, thereby to supply clinics with experimental andtheoretical basis, to provide a new way for developing new, low-toxic, and moreeffective drugs against glioma.Methods: After PCPS’ effect the growth inhibition rate of C6glioma cell line inrats was detected by MTT assay.60SD rats were randomly divided into5groups:normal control group, negative control group, carmustine group, PCPS group(200mg/kg), and PCPS group (400mg/kg). The effect of PCPS on tumor weredetected2weeks after tumor strains transplantation, the measurement of tumor wascalculated by the formula: volume=a2bπ/6(a: minor axis, b: major axis). Theantineoplasmic activity of PCPS was calculated by formula: Inhibition rate (%)=1-(average tumor volume of treated group/average tumor volume of negative controlgroup)×100. The mean survival time (MST) of each group was determined byrecording the mortality daily for60days, and the survival times of the treated group(T) were compared with those of the control groups (C) using the followingcalculation: increased life=MST of treated group/MST of negative control×100. Theweight of rats for each group was determined at7days before modeling,1day afterdrug administration,7days after drug administration, and14days after drugadministration; spleen and thymus were excised14days after drug administration.Spleen index was expressed as the thymus weight relative to body weight. The levelsof SOD, CAT, and MDA were detected by spectrophotometer and TBA, by which to detect the effects of PCPS on biochemical indicators. The difference between controlgroup and experimental group was detected by t-test, significant if P<0.05.Results: Compared to negative control group, PCPS has significant inhibitoryeffects on C6glioma cell line while concentrated at400μg/mL, the higherconcentration, the higher inhibitory rates,27.01%at50μg/mL,47.71%at100μg/mL,59.41%at200μg/mL,86.16%at400μg/mL. Smaller tumor volume appeared inpositive control group and PCPS group, and significant difference existed in treatmentgroup and negative group (bP<0.05andbbP <0.01). The mean survival time was22.49days in negative group,44.52days in carmustine group,32.48days and42.37days in PCPS groups (200mg/kg,144.44%;400mg/kg,188.4%),bbP <0.01intreatment and negative control group. Compared to normal control group, the weightdecreased significantly7days after the rats were inoculated C6gioma cells,aaP <0.01.In carmustine treatment group the weight of rats increased continually in low growth,especially at14daybP<0.05compared to negative control group, while by the daythe weight between PCPS (400mg/kg) group and negative control group,BP<0.05,238.87g and223.4g respectively. Compared negative control group with normalcontrol group the index of thymus and spleen significantly decreased,aP<0.05andaaP <0.01. Compared to the more negative, after carmustine treatment, thymus indexkept in original level, spleen index decreased rapidly,bbP<0.01. After PCPS treatment,these two indexes were nearly to those in normal control group. The levels of AST,ALT and ureophil in negative control group increased significantly compared withnormal control group (aP<0.05andaaP <0.01). These three indexes increasedcontinually in carmustine group, almost back to the normal in PCPS group. Comparedto normal control group, the activity of SOD and CAT significantly decreased, butMDA increased in negative control group. It was observed that in carmustine groupthe oxidation resistance was so worsened that the level of SOD, CAT, and MDAdropped to3.54,16.01,6.45respectively. These three parameters nearly backed to thenormal after the rats were given PCPS at400mg/kg, P <0.05. Conclusion: in this study, it was proved that PCPS has stronger antitumoractivity in vitro and in vivo, and its effects closely associated with inhibiting tumorgrowth directly, improving tumor rat’s immune and oxidation resistance, yet itsmolecular basis is still uncertain. |
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