An Experimental Study Of The Effect Of Therapy Of Warming Yang To Relieve Fluid Retention On The Differential Gene Expression In Pleura Of Rat With Hydrothorax

Objective:Genechip technology was used to measure changes in RNA expression, affected by therapeutic method of warming Yang to relieve fluid retention, of pleura in rat with X-carrageenan-induced pleural effusion.Methods:Male Sprague-Dawley rats were allowed to acclimate for3days before use, and then were randomly divided into group A and control group. Rats in group A were anesthetized with pentobarbital sodium, followed by the intrapleural injection of1%λ-carrageenan solution. At24h after the injection with carrageenan, the chest of rats was x-rayed, and the animals with a significant pleural effusion were determined by computed radiography. These valid rats were further divided into treatment group with intragastric administration of Chinese herb decoction (5ml/kg,1time per day) as well as model group with saline (5ml/kg,1time per day). Rats in control group were given saline (5ml/kg,1time per day). Four days later, the animals were euthanized by bleeding from the abdominal aorta under deep anesthesia with chloral hydrate. The parietal and visceral pleura of three rats from each group were obtained and the differential gene expression was analyzed using Agilent genechip.Results:In the parietal and visceral pleura, the differential gene expression between model and control groups as well as between treatment and model groups was associated with several types of biological processes and signaling pathways. In the parietal pleura, the therapy of warming Yang to relieve fluid retention could conversely regulate differential expression of16genes caused by X-carrageenan, including Fgd4, Mef2a, Fhl3, Dyrk2, Slc24a6, Nme7, Pbrml, LOC498368, Tp53i11, Rcan3, Hapln4, Abil, Hlx, Pold2, Klk11and Fkbp5. Moreover, in the visceral pleura, this treatment could conversely regulate differential expression of42genes affected by λ-carrageenan, including Rhoa, RGD1306349, Nsdl, Figf, Brd4, Crebbp, Sos2, Irf2, Brd2, Tspyl2, Eif4g2, Lcor, Ksrl, Slc25a27, Ptrf, Efempl, Isyl, Sf3a3, Msl2l1, Myo9a, Papolg, Vasp, Pdcd7, Zbtbll, RGD1559904, Csnklgl, Leng8, Ddx10, Arhgef3, Smg6, Zfat, Tm9sf3, Otud5, Chd6, RGD1563319, Cyb5r3, Gpr135, Polr3gl, Kcna5, Ugp2, Acadll and Btbdll.Conclusion:In our previous study with a Guinea Pig model of hydrothorax, we demonstrated that the therapeutic method of warming Yang to relieve fluid retention can facilitate recovering from pleural effusion. Our current study found that this therapeutic effect might be related to multiple biological processes and signaling pathways. The results also indicated that this therapy can down-regulate Mef2a, Dyrk2and Tp53i11expressed in parietal pleura and then reduce inflammation. Moreover, in visceral pleura it can down-regulate Rhoa, Figf, Brd4, Crebbp and Brd2, and up-regulate Ksrl and Slc25a27, which was associated with alleviative inflammatory injury.