The Silymarin Purification And Its Active Research In Milk Thistle Shell

Silymarin belongs to the compositae, it contains a natural flavonoids in milk thistle plants and seeds, which was the highest in seeds, with a wide range of applications in various industries and fields. In recent years, the study of silymarin in the laboratory mainly studies of silymarin main monomer compound silibinin separation and purification as well as physical activity, but for the other monomer compounds, especially water fly thistle Pavilion, silymarin Ning study is relatively small, at the same time, in recent years, studies in the extraction process silymarin conventional solvent extraction method, the extraction solvent is needed and more, the cost is very high, while the pollution of the environment is large, and for silymarin physiological function studies focused water fly with thistle Pigment For the protection system for the study of liver function hotspot of activity of very few other features. More of these issues are in the technology as well as research the physiological activity of hindering the development of silymarin, so build convenient and efficient extraction methods and separation and purification of the way in silymarin research, and improve research in other physiologically active areas has a very important practical significance. The main conclusions are as follows:1, Silymarin seeds have seeds and seeds shell separation of silymarin shell of silymarin enzymatically assisted extraction, silymarin optimal extraction conditions, the application of cellulose enzyme assisted extraction silymarin shell silymarin, enzyme concentration for the 30U/mL, reaction temperature is 45 ℃, reaction time was 120min, pH value of 4.5, after enzyme inactivation, reflux extraction 1h, silymarin extraction rate of 7.86%, compared with the same period the rate of traditional solvent extraction method improved 74.67%.2, The application HPD-600 macroporous resin and 200-400 mesh silica gel silymarin major monomeric compound isolated and purified, respectively, with 70% ethanol solution and petroleum ether-ethyl acetate solution was eluted using infrared spectroscopy, liquid chromatography-tandem mass spectrometry techniques (LC-MS-MS) and nuclear magnetic resonance spectroscopy to identify the structure of silymarin monomer, the results are as follows:petroleum ether-acetone 2:1 part to elute an flavonoids, identified as Douglas fir Su, from petroleum ether-acetone 1:1 part to elute an flavonoids, identified as silymarin Ning, petroleum ether-acetone 1:2 eluted three flavonoids by identification are silibinin and silychristin.3, Silymarin antioxidant activity in vitro and in vivo studies, then antioxidant test results indicated that different radical scavenging superoxide anion radical scavenging experiments, hydroxyl radical scavenging rate, the silychristin and free radical scavenging activity of silymarin Ning are the highest antioxidant activity of silibinin relatively low compared. In vivo antioxidant testing process, silymarin each dose of serum T-SOD, liver T-SOD, serum T-AOC, hepatic T-AOC, serum CAT activity, liver CAT activity, serum GSH-Px and liver GSH-Px content gradually increased, while serum MDA and MDA content in liver test cycle varies gradually decline, the test results showed that:each dose of silymarin can increase the class content and activity of antioxidant enzymes in serum and liver, reducing lipid peroxidation product vivo content, thereby increasing the antioxidant capacity of biological body.4, Silymarin immunomodulatory activity at the end of the test, liver ACP activity of each dose group, serum ACP activity, lysozyme activity, nitric oxide content, spleen index, stalls clearance index, phagocytic index and thymus index with the test cycle extend gradually increased in a dose-dependent growth. The results showed that:silymarin high dose group can increase the spleen index and thymus index, blood ACP activity, ACP activity of the liver and lysozyme content, thereby enhancing phagocytosis of other substances and ability of phagocytic cells to absorb, and silymarin high dose group significantly increased in mice carbon clearance index (κ) and phagocytic coefficient (α, therefore, silymarin high dose group can enhance the biological organism nonspecific immune function; silymarin high dose group can raise blood nitric oxide content, indicating that silymarin by activated macrophages play a non-specific immune function, lymphocyte involvement in the complex immunization activities.5, Silymarin anti-fatigue activity, at the end of the test, compared with the control group, the experimental weight each dose group and organ weight was no significant difference (P> 0.05), the anti-fatigue test, each dose of silymarin Mice climbing pole time, swimming time, liver glycogen and muscle glycogen content increased, while BLA BLA BUN and serum urea nitrogen content gradually reduced, at the same time, silymarin liver of mice (AST, ALT and white protein), bile duct function (ALP and total bilirubin), heart health scale (LDH), muscle function (CK), renal function (creatinine and uric acid), nutritional status (total protein), and lipid levels (TC and TG) There were no significant side effects. Based on the above analysis, silymarin for movement mice fatigue process played a very beneficial effect, anti-fatigue damage effect is very significant, indicating that the muscle cells long sports injury has a protective effect, and can be associated with increased activity and energy metabolism within skeletal muscle movement, promoting cell aerobic metabolism. And with silymarin high-dose group produced the best anti-fatigue effect. Paraffin movement before and after the show, silymarin to damage the fine structure of cardiac and skeletal muscle in mice caused by prolonged exercise due relieve effect. But not very clear mechanism for its ease fatigue, need to be further studied.