| Background Digestive tract reconstruction style is closely related to the postoperativ e recovery, this study compared differ styles when performance the procedure includi ng traditional ileorectal anastomosis(T-IRA), antiperistaltic side to side ileorectal anast omosis(SS-IRA) and antiperistaltic cecoproctostomy(ACP) through a retrospective anal ysis,seeking a more reasonable method to resolve intractable postoperative complicati ons such as diarrhea and constipation recurrencesMethods A total of 65 patients underwent TAC for refractory idiopathic STC.Twenty patients were treated using traditional SE-IRA,22were treated using antiperistaltic S S-IRA,whereas 23 patients were treated using ACP. Patients were evaluated at 3 an d 6 months as well as at 1 and 2 years after surgery.Both groups were compared fo r patient characteristics,perioperative data and quality of life. Wexner Incontinence sc ale (WIS) and Gastrointestinal Quality of Life Index (GIQLI) were adopted for evalu ating postoperative recovery.Results 1.A11 groups were comparable with respect to general patient characteristics, disease severity and post-operative complications. Fewer than 30% of all patients rep orted substantial dissatisfaction with surgical outcomes in both the groups. The SS-IR A group was associated with a lower postoperative WIS (p< 0.05) and a better GQ ILI(p< 0.05) than that of the SE-IRA group during early followup examinations.2 Compared to ACP procedure,SS-IRA showed no significant difference in c onstipation relief and quality of life improved(p> 0.05),even the former had a more common defecation frequency(p< 0.05)3Diarrhea is the major complication of IRA procedure, fortunately it mainly improved 12 months later after surgery. Postoperative bloating and abdominal pain i s more common in CRA procedure, No patient need operation again during the flow ing up periods.Conclusion In this study, SS-IRA was superior to traditional SE-IRA for the treat ment of STC with respect to post-operative outcomes, and especially during early follow-up.ACP brought a better bowel movements,however it is related with a risk of recurrence and abdominal symptoms.Objective Protoemics has been one of the most important approaches in the researchs of disease related pathogenesis,biomarker screening,target to pharmacological action and gene interfernence.The present study was proposed to separate the global protein in dynamic and normal colonic tissue of slow transit constipation in 2-D profiles,and identify them with MS.Methods The procins of five dynamic colonic tissue and their coupled normal full-thickness colonic tissue were extracted and separated by 2-DE.Proteins on 2-DE were visualized by Image Master 2D 5.0 version software.Then the partial differential expressions spots were cut for in-gel digestion.The peptides were identified by Mass spectrometry and the data were searched against database.Results Five couples of 2-D with ideal resolution and reprodueibility were obtained,274± 30and 212±24 spots detected in normal and dynamic disorder colonic tissues, respectively.There were more than 76 differential protein spots that were significantly differentially,and 20 spots were picked for identification.At last 15proteins were identifiedConclusion 1 The standard,reproducible 2DE patterns of dynamic and normal eolonic tissue from the patients of STC are established.2. The 2DE patterns of dynamic group and control group and 15 proteins were identified by mass spectrometry.These may be associated with the pathogenesis of STC;However the concrete relationship of the identified proteins with STC is need more research. |
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