The Study Of Fermentative Condition And Chemical Enhance Technology Of B.R3in Biohydrogen Production System

The anaerobic fermentation biohydrogen production can make use of the high concentration organic waste and biomass (such as complex solid waste, sewage sludge and so on) as raw material to produce hydrogen. This technology has caught more and more focus since it can operate easily, produce hydrogen efficiency and sustainly, but the most important is that this technology can not only solve the environment problem but also produce hydrogen.This paper investigated the hydrogen production efficiency of Biohydrogenbacterium R3sp.nov (B.R3in single) in batch condition and the promotion of different fermentation condition (including initial pH, temperature, biomass etc.), organic nitrogen, inorganic nitrogen, phosphate and metal ions on hydrogen production efficiency and cell growth to it according to the principle of anaerobic fermentation biohydrogen production. We also investigated the efficiency of B.R3in continuous stirred tank reactor (CSTR) which makes molasses as substrate. The results are as followed in simply.The results indicated that pH and temperature had a noticeable effect on the cell growth and hydrogen production. The dry cell weight and hydrogen production yield got the maximum of0.63g/L and34.2mmol/L respectively when the initial pH was5.5. The final pHs in the culture were always kept at3.0-4.0. Temperature affected the maximum cell growth and specific hydrogen production ration (SHPR) and they got the maximum of0.66g/L and1.01mol H2/mol glucose respectively at the temperature of30℃. Whether the pH and temperature are lower or higher, hydrogen production and biomass will be inhibited gradually.The biogas, hydrogen production and the hydrogen concentrations were187.5mol/L,89.28mol/L and47.62%respectively when was used the glucose as subtract. The hydrogen concentrations were36.92%and43.75%while the biogas and hydrogen production yield were relatively lower than those when the milk and maize flour were used as subtract. This indicated that the milk and maize flour have the capacity of hydrogen production although the hydrogen production rations were low since they have the rich carbonate. Many reachers’ investigation shows that the carbohydrate has high hydrogen producing ablitity. While the cellulose must be pretreated by acid or alkali so they thought it’s not suit for hydrogen production. But China waste huge amount of cellulose every so we think it is valuable because this can treat the waste and produce hydrogen at the same time.The reacher of bacteria in continuous condition is less than that of mixed culture relatively. The B.R3can produce hydrogen continuously in the right condition. The CSTR was set up at the initial COD of4kgCOD/L-d and it dropped to3kgCOD/L-d at the23th day while it dropped to2kgCOD/L·d at the49th day. The hydrogen production, concentration and end liquid fermentation fluctuated with the operation of CSTR. The hydrogen production and concentration increased when the CSTR operated at1th～23th day and the hydrogen production yield got the maximum of24.904L/d. The hydrogen production yield and concentration kept stable and got the maximum of26.39L/d at the28th day. The hydrogen production yield and concentration dropped to the minimum of0.90L/d at the51th day and they were increasing slowly with the NaHCO3and the operation of CSTR.The performance of organic nitrogen resource to B.R3on cell growth and hydrogen production was better than that of inorganic nitrogen resource. The yeast powder was most beneficial to promote cell growth and hydrogen production among all those nitrogen resources. So it’s essential for B.R3to dose organic nitrogen (epically yeast powder) to promote the hydrogen production.K2HPO4can promote the hydrogen production and maintain the pH in medium of B.R3well. When the initial pH was5.5, the temperature was30℃and the yeast power as nitrogen provider, the biogas production yield, hydrogen production yield and hydrogen production ration got the maximum of4960mL/L·culture,2107.5mL/L·culture,1.93mol H2/mol glucose when the concentration of K2HPO4was1.5g/L. The pH in medium always maintained3.0-5.0since the K2HPO4can buffer the medium well.The phosphate buffer solution (PBS) can promote the hydrogen production and buffer the pH in the medium efficiency to B.R3The biogas and hydrogen production yield and average hydrogen production ration got the maximum of3860mL/L·culture,1832.7mLH2/L·culture and2.6324mmol H2/g·cell·h when the concentration of PBS was0.15mol.Compare the promotion to B.R3of NH4HCO3, NaHCO3, Na2CO3, K2HPO4, KH2PO4, NaH2PO4and Na2HPO4comprehensive, the hydrogen production yield and hydrogen concentration got the maximum of1978.56mL/L·culture,44.1%,2160.9mL/L·culture and45.8%when Na2HPO4and K2HPO4was dosed in medium.The hydrogen production yield got the maximum of205mL/L·culture,315mL/L·culture,281mL/L·culture,192mL/L·culture,144.2mL/L·culture respectively at the concentration of CoCl2at0.05mg/L, Fe粉at200mg/L, Fe2+at40mg/L, Cu2+at0.03mg/L, Zn2+at0.2mg/L.