| The root-knot nematode is one of the most widely distrbuted and heaviest hazard plant pathgenic nematodes. The main methods to control the disease are chemical, biological and physical control with the most feasible method being biological control, but the mechanizm of Paecillomyces lilacinus on root-knot nematode still uncertainty. In this paper, GFP constructed strain of Paecillomyces lilacinus were used to observe the infection mechanizm, combined with SEM observations, the blending protease content of root-knot nematode and Paecillomyces lilacinus was tesed, then hoping to find out the interaction between them. At the same time the relation between disease severity and microbial factors or non-microbial factors were investegated.The effect of metal ions on Paecillomyces lilacinus was studied, to offer theoretical basis for the disease of root-knot nematode. The main results as follow.In this paper, four strains of Paecillomyces lilacinus were transferred to GFP strains, there are459strain, P strain,20-7strain and618’strain. All of them could see fluorescence light under fluorescence microscope, the fluorescence light of459and P GFP transferred strains were weak, the fluorescence light other GFP transferred strains were strong. Four transferred strains were inoculated continuously for four times, the fluorescence light of459and P GFP transferred strains a mostly dispersed, but the fluorescence light of20-7and618’GFP transferred strains still strong. The conversion efficiency of20-7and618’GFP transferred strains were high, there are to90%.The same volume of Paecillomyces lilacinus combined with different volume root-knot nematode eggs, the initial protease content different little, inoculated for six days the content of protease showed down trend, inoculated for eight days, the content of protease down rapidly. The protease content of3mL Paecillomyces lilacinus mixture and lmL root-knot nematode eggs mixture less than the protease content of3mL Paecillomyces lilacinus mixture and2mL root-knot nematode eggs mixture less than the protease content of3mL Paecillomyces lilacinus mixture and3mL root-knot nematode eggs mixture. Initially The protease content of3mL paecillomyces lilacinus mixture and1mL root-knot nematode eggs mixture highest for the first day, changed by time, the protease content reached to lowest for the eighth day, the first day to the sixth day, the protease content of the mixture changed no obviously, but the last two days changed obviously. The protease content of3mL Paecillomyces lilacinus mixture and2mL root-knot nematode eggs mixture changed slowly from the first day to the sixth day, from the sixth day to the eighth day down obviously, to the eighth day the protease content reached to lowest. The protease content two mixtures above changed similarly. The protease content of the three mixtures all down by time, due to every treatment add to different eggs the protease content of the three mixtures were different initially. The more eggs mixture the higher protease content.Under dissecting microscope, the eggs hatch out for the second day, a large number of larvae appeared, the number of larvae increased gradually with time changed, the number of the larvae reach to climax for the fourth day. The eggs which unhatched the shape inside changed a lot, the eggs changed to second division four the fourth day, and inside the division eggs a lot of vesicles appeared, which had the similar size. After a week, the eggs which unhatched appeared vesicles, after fourteenth day the eggs which vesiculated could not see in the mixture.The main bacteria of the rhizosphere soil in the root-knot nematode disease area were Pseudomonas sp.. Uncultured Sphingobacterium sp., Bacillus sp., Acinetobacter genomo sp. and Flavobacterium sp.. The number of Pseudomonas sp. was most, reached to70%, the number of Bacillus sp. reached to17%, the number of Flavobacterium sp. was10%. The number of Uncultured Sphingobacterium sp. and Acinetobacter genomo sp. was less.The main fungi of the rhizosphere soil in the root-knot nematode were Chaetomium sp., Paecilomyces sp., Penicillium sp., Fungal endophyte sp. and Cladosporium sp.. The number of Penicillium sp. was most, the second is Chaetomium sp., the third was Paecilomyces sp.. The main actinomycetes of the rhizosphere soil in the root-knot nematode disease area were Streptomyces sp., Micromonospora sp. and Madura actinomycetes sp..The total content of three different disease rate of rhizosphere soil had a little difference, the content of organic matter in the middle disease soil was higher, the content of available P and available K in the heaviest disease of rhizosphere soil were higher, there were309.92mg/kg and277.29mg/kg. The PH value of soil adopted present was acid, the PH value of the heaviest disease was smallest (4.24), the PH value of the no disease soil was nearly to neutral.In this paper three metallic ion had effect on618’strain and transferred strain of paecillomyces lilacinus. The result indicated that the effect of Cu2+ion on618’significantly, when under the concentration of1×10-6、1×10-5、1×10-4, the strain grow rapidly, the strain diameter was different. When above the concentration of1×10-3mol/L, the strain stopped growing. The affection on618’strain and transferred strain of Cu2+was that:the three concentration of1×10-6、1×10-5、1×10-4increased the growth of618’strain and transferred strain, under the first concentration and the second concentration the two strains grow rapidly, but the growth rate of orginal strain was higher than transferred strain’s, the affection of concentration of Mg2+ion on618’strain and transferred strain was a little difference, the best concentration of Mg2+ion for the growth of618’strain was0.005mol/L, when the concentration low to0.1mol/L, the strain grow well, above the concentration, the strain grow slowly, while the concentration up to0.6mol/L, inhibitory rate reached to100%, under the concentration of0.1mol/L, inhibitory rate reached to50%more or less. The affection of the ion on618’strain and transferred strain had a little difference, during the concentration of0.4-0.6mol/L, both the strain could not grow, the concentration between0.1-0.4mol/L, the growth almost the same, under the0.1mol/L, both the strain were grow well, the growth rate showed up trend, above50%, but the growth rate of transferred strain higher than the original strain. The affection of all concentration of Al3+ion on strain growth changed a little, the concentration of1×10-6mol/L and1×10-5mol/L were benefit for growing, the strain diameter were bigger, by the concentration increasing, the strain grow slowly, the strain diameter were small, above the concentration of1×10-4mol/L the strain grow slowly, the strain stopped growing when the concentration reached to5×10-2mol/L. The concentration of Al3+ion effected on618’ strain and transferred strain was1×10-6～1×10-4mol/L,618’ original strain and transferred strain both showed down trend, under high concentration, the growth rate of the two strain made a little distinguish. |
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