Analysis Of Differentially Expressed Gene Related To Lipid Metabolism Between Adipose Tissues And Longissimus Dorsi Muscle In Cattle

Microarray technologies, which can measure the expression of thousands of genessimultaneously, are useful in understanding global gene networks and in identifying novel genesand functional gene classes. Lipid deposition patterns are associated with the economic value ofbeef. Therefore, it is desirable to select lipid deposition patterns in beef cattle that improve meatquality. However, little is known about the genes involved in different adipose tissue deposition.The objective of this study was to search for candidate genes related to sites of fat deposit. Toprovide theoretical basis for improving meat quality traits in cattle breeding process, geneontology and pathway analysis further validated differentially expressed genes identified byanalysis of the array.1、Microarray analysis was performed to create a gene expression profile of subcutaneous andabdominal adipose tissues in Yanhuang cattle. The data were analyzed using Significance Analysisof microarrays to identify differentially expressed genes.330genes were differentially expressedbetween subcutaneous and abdominal adipose tissues. Based on Gene ontology analysis conductedon the free web based Molecular Annotation System3.0,22genes were related to growthdevelopmen19were genes related to lipid metabolism and9genes were related to energymetabolism. Gene set enrichment analysis was used to select pathways showed that gene sets wereup-regulated in subcutaneous adipose tissues involved in oxidative phosphorylation andsphingolipid metabolism gene sets were up-regulated in abdominal adipose tissues involved infatty acid metabolism and peroxisome proliferator-activated receptor gamma signaling pathway.2、The data were analyzed using significance analysis of microarrays to identify differentiallyexpressed genes,5754genes were differentially expressed between adipose tissues andLongissimus Dorsi Muscle. Based on Gene ontology analysis,5754differentially expressed genesinvolved in147biological process,69cellular component,68molecular function. Based onpathway analysis,28pathway involved in differented expressed genes.368genes weredifferentially expressed among subcutaneous adipose tissues, abdominal adipose tissues andLongissimus Dorsi Muscle.332genes were involved in biological process.90genes wereinvolved in cellular component and228genes were involved in molecular function. Based onpathway analysis,86pathway involved in differented expressed genes. 3、The mRNA expression levels of PPARγ, FASN and ACADM in adipose tissue andLongissimus Dorsi Muscle were compared between Yanbian Yellow cattle and Yanhuang cattle.The relative expression level of ACADM gene in different adipose tissues and Longissimus DorsiMuscle were not significant in two different cattle species.but this difference was not statisticallysignificant. FASN mRNA expression levels in subcutaneous fat and abdominal fat in Yanhuangcattle were significantly higher than that in Yanbian Yellow cattle (P <0.05). The PPARγ mRNAexpression level in subcutaneous fat in Yanhuang cattle was higher than that in Yanhuang cattle (P<0.05), But The expression levels of PPARγ gene in perirenal fat of Yanhuang cattle was lowerthan that in Yanbian Yellow cattle (P <0.05). In addition, the expression levels of PPARγ genewere high in abdominal fat in both cattle species. Our results make a basis on screening majorgenes related to lipid metabolism in cattle.