The Pathological Classificasion Analysis Of Breast Microcalcifications And The Research Using Fourier Transform Infrared Spectroscopy

Calcifications are very important diagnosis information in breast tumor.Now we can distinguish between benign and malignant lesions by the analysis of calcifications.Recent reseach tells us,the characters of breast microcalcifications are maybe related to prognosis of patients.The mechanism of breast microcalcification forming is not clear now. Calcifications have been known as deposition of tissue necrosis.In particular, the issue as to whether it is a sign of degeneration or of an active cell process is still unresolved. In earlier studies, breast tumors were shown to demonstrate both amorphous and crystalline deposits.The infiltrating ductal carcinoma cases with breast microcalcifications were chosen as research obiects. The following work was carried out:1. we classify the infiltrating ductal carcinoma cases with breast microcalcifications by the the characters of pathology;2.we build the study model of breast microcalcifications by FTIR;3.we analyse the breast microcalcification focus,the necrosis around the microcalcifications and the tumor tissue around the microcalcifications by FTIR. The following main results were obtained:1. The pathological classificasion analysis of140Infiltrating Ductal Carcinoma cases with breast microcalcifications:According to the microcalcifications in lumen or out of lumen,and the microcalcifications with coagulation necrosis or without coagulation necrosis,we classify the breast microcalcifications in infiltrating ductal carcinoma into three types:type I a are microcalcifications in lumen with coagulation necrosis,type I b are microcalcifications in lumen without coagulation necrosis,type Ⅱ is out of lumen.70%of cases are type I a,20%of cases are type I b,and10%of cases are type Ⅱ(p<0.01).51%of the cases are Invasive ductal carcinoma(without DCIS),while49%of cases are DCIS.100%of cases with DCIS are in lumen.2. We build the study model of breast microcalcifications by FTIR:After statistical analysis, the calcified area (A), necrotic zone (B zone), calcified the cancer tissue around the area (C area) a few basic characteristics peak position is basically the same, indicating that the basic groups of substances contained in three districtsconsistent absorption signals of intracellular proteins, nucleic acids, and CHAP. Through the comparison of the peak height and peak area, the peak intensity of calcified the cancer tissue around the area (C area) amide bands are larger than the other two districts, a statistically significant difference between the two groups, so the Fourier transform infrared spectroscopy applied to breast cancer the microcalcifications research is feasible.3.By Fourier transform infrared spectroscopy of breast cancer cases of microcalcifications calcification, calcification around necrosis, calcification around the cancer tissue between the infrared spectrum:found in breast microcalcifications, calcification, areas of necrosis and calcification aroundcancer tissue area contained the same basic ingredients, showed that the calcification area around the cancer cells have been CHAP ingredients for protein and CHAP, CHAP ingredients necrotic area, which showed that breast cancer cells can synthesize CHAP, the lumen CHAP is likely to be stacked to form after the death of a large number of breast cancer cells. The study found that calcification area, areas of necrosis and calcification around the cancerous tissue area CHAP content with a declining gradient, which may be closely related to the special environment and ductal breast cavity.The results of the study show that:the breast microcalcifications initially formed around ductal breast cancer cells, the catheter lumen calcification breast ductal around cancer cells closely related. This will be the future of breast microcalcifications formation mechanism studies provide a basis to provide a theoretical basis for the clinical diagnosis of breast cancer as well as breast microcalcifications, Fourier transform infrared spectroscopy and will contribute to the promotion to the clinical diagnosis of breast cancer and study.