Study On The Relationship Between Aerobic Vaginitis In Pregnant Women And Pregnant Outcome And Group B Streptococcus Infection In Mother And Neonates

Aerobic vaginitis is common vaginitis of women.Aerobic vaginitis in pregnant women can seriously affect the quality of perinatal care, cause many adverse effects on pregnancy. Studies have found that group B streptococcus is common vaginitis pathogen of aerobic vaginitis, but aerobic bacteria during pregnancy on pregnancy outcome and gene effects and maternal and child group B Streptococcus serotype type is not reported in China. Therefore, we collected150cases of different maternal during the2010-2012in our hospital of vaginal secretions, analysis of flora distribution and pregnancy outcome and correlation, and the mother-baby pairs and gene of GBS strain serotype type.Part I The relationship between aerobic vaginitis in pregnant women and pregnant outcomeObjective To investigate the vaginal flora distribution and its correlation with pregnancy outcomes of aerobic vaginitis women of pregnancy in different periods.Methods We choiced150cases of aerobic vaginitis in pregnant women from July2010to July2012in our hospital routine prenatal obstetric outpatient service.50cases of first trimester group (gestational age<13weeks),50cases of second trimester group (13weeks<gestational age<28weeks),50cases of third trimester group (gestational age≥28weeks). And we also choiced100cases of normal pregnant women during the same period in the hospital routine prenatal obstetric outpatient service as control group. Vaginal Secretion Smearing and Culturing. Then we followed-up pregnant outcome including gestational weeks, the Mode of delivery, rupture of membranes, chorioamnionitis, puerperal infection, fetal distress, postpartum hemorrhage, neonatal weight, Apgar score and infection in neonates.Results1. The mainly bacteria in aerobic bacterium vaginitis infection of pregnant women were group B streptococcus, e. coli bacteria, staphylococcus ball bacteria and enterococcus. There was no statistically significant difference between each pregnancy group(P>0.05). 2.There was statistically significant difference between aerobic bacterium vaginitis women and normal pregnant women in incidence of premature rupture of membrances(P<0.05);There was no statistically significant difference among different aerobic bacterium vaginitis pregnant women in the incidence of premature rupture of membranes(P>0.05). There was statistically significant difference between aerobic bacterium vaginitis women and normal pregnant women in incidence of chorioamnionitis (P<0.05); There was no statistically significant difference among different aerobic bacterium vaginitis pregnant women in incidence of chorioamnionitis (P>0.05). There was statistically significant difference between aerobic bacterium vaginitis women and normal pregnant women in the incidence of postpartum hemorrhage(P<0.05); There was no statistically significant difference among different aerobic bacterium vaginitis pregnant women in incidence of postpartum hemorrhage (P>0.05).3. There was statistically significant difference between aerobic bacterium vaginitis women and normal pregnant women in the incidence of and infection in neonates(P<0.05);. neonatal weight, Apgar score of newborn of aerobic bacterium vaginitis women were lower than normal pregnant women(P<0.05).4. After treatment,There was statistically significant difference between the treatment group and non-treatment group in incidence of premature rupture of membranes(P<0.05).In pregnant women, The bacterial culture of aerobe incidence of chorioamnionitis of102cases of treatment group was4.9%, lower than incidence of chorioamnionitis of non-treatment pregnant women and the difference was statistically significant (P<0.05). The non-treatment pregnant women had significantly higher incidence of postpartum hemorrhage than treatment women(P<0.05). while the incidence of fetal distress and preterm birth and puerperal infection in the two groups, there were no statistically significant difference (P>0.05).5. There was statistically significant difference between untreated pregnant women and treatment group in the incidence of infection in neonates(P<0.05); neonatal weight, Apgar score of newborn of untreated pregnant women were lower than treatment group (P<0.05). Conclusion1. The mainly bacteria in aerobic bacterium vaginitis infection of pregnant women were group B streptococcus, e. coli bacteria, staphylococcus ball bacteria and enterococcus. There was no statistically significant difference between each pregnancy group.2. Aerobic bacterium vaginitis can cause many adverse effects such as:premature rupture of fetal membrane,chorioamnionitis,postpartum hemorrhage. After treatment, Pregnant outcome woulde be improved.Part Ⅱ:Analysis of serotype and Genotype of Group B streptococcus in pregnant women and their newbornsObjective To investigate mother son paired GBS serotype strains, and DNA fingerprint are consistent, in order to be early prevention, early treatment, improve the outcome.Methods We selected of vaginal secretion culture for group B streptococcus of pregnant women and their newborns oropharyngeal secretions training GBS as the research object, in selecting strains, used immune double diffusion method and compare serum type of credits and mother son paired GBS serotype strains. Through RAPD, We studied GBS genotype.Results1. We used immune double diffusion method to analyze serotype GBS bacteria of72strains.9serotype were isolated, including Ⅱ and Ⅲ main serotype, another three GBS inseparable type strains.2.12cases of neonatal GBS antigen and its matching mother GBS antiserum precipitation line corresponding to precipitate line total integration formed a smooth curve. It showed that they had the same both GBS main antigen components, a quite close relationship, namely the strains with high similarity.3. Through RAPD, We studied of18kinds of GBS genotype.7random primers can be used in four of the article presents good polymorphism and stability, and can produce significant difference of the fingerprint. To analysis the genetic distance between the different strains.18strains of GBS genetic distance were frome0.082to0.531, the average distance of0.35,18strains of group B streptococcus4strains and13strains of genetic distance, the biggest0.358.1and7of the smallest genetic distance was0.011. GBS of different serotype strains had similar fingerprint, the same serotype strains had different fingerprint.Conclusion1. A total of nine serotype was isolated, of which Ⅱ and Ⅲ were main serum types. We also found that three strains of GBS were inseparable type. GBS production strains between pregnant women and their babies were high similarity.2. GBS of the same serotype strains had different fingerprint,different serotype strains had similar fingerprint.