Catgut Implantation At Acupoints For Th1/Th2, Treg/Th17in Rat’s Nasal Mucosa Of Allergic Rhinitis

Object:Allergic rhinitis (AR) is a common frequently-occurring disease in otolaryngology, which is one of the main factors leading to asthma and has become a global public health impact. ZhangQinXiu professors consider that the pathogenesis of this disease is the lung, spleen, kidney disorders, Yang failure is basic, pathogeny is actuating factors for this disease. To therapy AR, Professor Zhang uses catgut implantation at acupoints to benefit lung and spleen and to rise Yang and exorcising pathogeny which has been approved effectively in clinic. This topic will approve the effect of catgut implantation at acupoints for AR through the animal experiment and discusses the mechanism based on pathological morphology and immunology.Method:Health SPF SD rats were randomly divided into blank group (Ⅰ), AR model group (Ⅱ), AR western medicine group (Ⅲ), AR false catgut implantation at acupoint (Ⅳ), AR catgut implantation at acupoint (Ⅴ). We made AR animal model according the method of improved ovalbumin injection and nasal mucosa stimulation. Every group received different therapies except blank group (Ⅰ). After last therapy day, we gave ovalbumin nasal mucosa stimulation,then we observed and record the AR symptoms to compare the symptom records. We killed SD rats and collected rats blood, septum mucosa to observe rat nasal mucosa pathological change under electron microscopy, to detect rat serum IgE content using ELISA method, to detect nasal mucosa tissue IFN-y, IL-4, TGF-β1, IL-17, SP expression using immunohistochemical method. We used the data SPSS17.0software for statistical analysis.Outcome:Catgut implantation at acupoints can obviously alleviate the symptoms allergic rhinitis (sneezing, runny nose, scratching) of AR rats. Compared with model group(Ⅱ), catgut implantation at acupoints can improve pathological changes of AR rat nasal mucosa,reduce IgE content in AR rat serum, reduce IL-4, TGF-β1, IL-17, SP content, increase IFN-γ content in the AR rat nasal mucosa. Catgut implantation at acupoints can reduce TGF-β1content AR rats in nasal mucosa.Conelus i on:This research established" allergic rhinitis rats model" by ovalbumin sensitization method. We operated Catgut implantation at acupoints in allergic rhinitis rats, and set up the control group (Triamcinolone acetonide nasal spray), then we found:1、The serum IgE and nasal mucosa SP, IL-4, IL-17, TGF-β1increased and nasal mucosa INF-y lowered significantly in allergic rhinitis rats, which means Th1/Th2and Treg/Th17immune disbalance of nasal mucosa is the most important pathogenesis in AR rats.2^’Catgut implantation at acupoints’can alleviate symptoms of AR rats.3^’Catgut implantation at acupoints’can reduce IL-4, TGF-β1, IL-17, SP content, increase IFN-γ content in the AR rat nasal mucosa to regulate Th1/Th2and Treg/Th17immune balance in AR rat nasal mucosa.4、Catgut implantation at acupoints’ and Triamcinolone acetonide nasal spray’ have similar effect at serum IgE and nasal mucosa substance P, IL-4, IL-17, TGF-β1、INF-γ in allergic rhinitis rats.