The Research Of TCM Vitality Theory And Effects Of Vitality Consolidation Treatment To Alzhermer’S Disease

Objective：Traditional Chinese medicine Qi theory is an important content of thetheoretical system of traditional Chinese medicine， which is based onthe philosophy ofprimordial qi theory， which mentioned some times inthe "Huang Di Nei Jing" and Nan Jing， and ancient physicians haveattached great importance to the prevention and treatment of diseases ofthe human spirit. it is the objective requirement of senile disease， chronicdisease prevention， that Emphasis on the theory of traditional Chinesemedicine research strength. at the same time， it is also the needs tochange from a medical to health care and prevention. This topic set out fromthe Chinese medicine Qi theory of its own context that developed byitself. has carried on the research and induction of the theory of traditionalChinese medicine literature and vigor vitality and reinforce academic schools.Combining the previous research of the group， take senile dementia anddegenerative diseases of the elderly as the breakthrough point to explore themechanism of strength with the Alzheimer disease relationships and cultivatea solid Prevention Act of senile dementia. With APP/PS1double transgenicmice as an animal model， to observe the Pei yuaneffective herbal tonic yuan Decoction in treating senile dementia and itsmechanism of action. Aimed at providing a theoretical basis and experimentalevidence for further more depth research from theory of TCM study， andvitality injury pathogenesis and elderly population strength loss research， thedevelopment research of the product and the system of health care in peiyuansolid methods， also about the mechanism of experimental study andanti-aging therapies and application of prevention and treatment of geriatric onthe basis of the peiyuan solid. Method：1、 Theoretical discussion：Collate the ancient and modern literature about the strength of Chinesemedicine， summarized the basic concepts of strength， the theoreticalconnotation of Medicine，generation，distribution，cognitive function andother content.Study of the formation and development of traditional Chinesemedicine theory vitality academic context，and the formation of Pei-Yuanconsolidate academic schools，the development and impact. To investigate thecorrelation between Qi and the human brain function，explain the relationshipbetween the strength damage and Alzheimer’s disease pathogenesis， aswell as Peiyuanpreventing senile dementia therapy.This paper considers the Qi theory of TCM has a distinctive strengthcharacteristics of Chinese，with a strong philosophical heritage，richnessin content and with enough practical movements. Ancient physicians enrichedand developed the theory of traditional Chinese medicine，vitality and vigorformed consolidate academic schools with the guidance of Qi theory in theHuangdi Neijing，and in the course of long-term fruitful clinical practice，which has accumulated valuable experience for human delay aging，prevention and treatment of age-related diseases.2、 Experimental study：Using APP/PSI double transgenic mouse model of dementia， with A βgeneration， deposited as target to study the role and mechanism of thetraditional Chinese medicine Nourishing strength Da Bu Yuan Jian which wasused for Reinforcing primordial qi for AD. Using morris water mazetest，step-down test，shuttle box test method，dark avoidance test to checkthe ability of learning and memory in mice. with electron microscopy andCongo red staining to test the effect of Dabu yuan Decoction on ADtransgenic mice pathological injury of hippocampal neurons. ELISA method to detect A β content in AD transgenic mouse hippocampal， WesternBlot method is mainly observed expression of Aβ generation and secretion ofenzymes degrading enzyme (α-secretase enzyme：ADAM-10， β-secretaseenzyme：BACE1， γ-secretase：PS-1， degradation enzyme：NEP， IDE)and its expression of upstream β-amyloid ‘precursor protein (APP)， byimmunohisto chemistry to detect pathological changes in the hippocampal AβAD model mice deposition.Results:Morris water maze test showed that compared with the control group，Mice in model group has longer total swimming distance and platform latencyand which were significantly (P <0.01)；compared with the model group，thetreatment group of mice on the platform latency and swimming distance weresignificantly reduced (P<0.01，P<0.05). Compared with the control group ofWestern medicine in donepezil， Dabu yuan decoct Chinese traditionalmedicine group showed no obvious difference about swimming platformlatency and total distance (P>0.05). Compared with the blank group， modelgroup mice’s number through the platform， the target quadrant timedecreased significantly and the first time reach for the original platform wasobviously prolonged (P<0.01)；compared with the model group，the numberthrough the platform and target quadrant time was significantly increased ineach drug-treated group mice，and the first time against the original platformwas significantly shorter (P<0.01，P<0.05)；Compared with western medicinedonepezil group，Da Bu Yuan Jian mice target quadrant time and the first timeagainst the time of the original platform was not significantly different (P>0.05)， the number of platform cross reducing significantly (P<0.05).Compared with the control group，the latency was significantly shorter and thenumber of errors increased significantly in model mice (P<0.01)；comparedwith the model group，the rest of each treatment group mice’s；latency were significantly delayed and error number decreased significantly (P<0.01)； compared with western medicine donepezil group， no significantdifferences in latency and error-free number of Da Bu Yuan Jian mice (P>0.05)， other behavioral experiments， such as passive avoidance experimentsshowed some similar trends. Pathological observation showed that the numberof senile plaques in hippocampus and areas were reduced in model mice，while after the treatment of western medicine and Da Bu Yuan Jian， the micehippocampus neuropathy reduced. ELISA test results showed that Da Bu YuanJian’s therapy can significantly reduce the soluble Aβl-40and Aβl-42concentration of soluble brain tissue. Western blotting results showed thatafter the treatment of Da Bu Yuan Jian， brain APP degradation waspromoted， increased expression ofNEP、IDE， while expression levels ofBACE1and APP were not affected.Conclusion1、Full of vigor and vitality，pulp filling and Shan fulled is the materialbasis of brain function； Qi deficiency， God not reservoir is theimportant link of senile dementia， and also is the pathological basis of seniledementia. Pei-Yuan consolidate， Xingnaoyizhi is the important therapies forAlzheimer’s prevention and treatment.2、 Peiyuan method can improve the abilities about learning andmemory of dementia mice.3、The mechanism of Peiyuan consolidate method in the treatmentof senile dementia was related to the decrease of hippocampal A β depositionand promotion of the degradation of A β.