The Research Of TCM Vitality Consolidation Treatment Theory And It’s Effects To Alzheimer’s Disease

ObjectiveVitality Consolidation Treatment has a very important impact on the formation and development of TCM treatment system. It plays an important role in a variety of diseases, especially of geriatrics. The ancient doctors attach great importance to this theory and interpret it much. This topic analyzes, concludes and summarizes the basic theory of Vitality Consolidation Treatment, discuss the relationship between Vitality Consolidation Treatment and the Origin Qi theory, study the mechanism of prevention and treatment of AD. APP/PS1 double transgenic mice is used as AD model to observe the effect of Buyuanqingnaokeli to AD, and the influence of PI3K/Akt pathway. And provide the the theoretical basis and experimental evidence to further theoretical research and clinical research of Vitality Consolidation Treatment. Method1.Theoretical discussion. Collate the ancient and modern literature about the Vitality Consolidation Treatment theory, summarize the basic concepts, theoretical connotation, generation, distribution, cognitive function and other contents. Clarify the theoretical basis of the relationship among the Vitality Consolidation Treatment and AD.2. Experimental study. APP/PS1 double transgenic mice is used as AD model, PI3K/Akt pathway is used as target, to study the effect of Buyuanqingnaokeli to AD. Morris water maze and diving platform as the ability of learning and memory. Western Blot to detect the main protein of PI3K/Akt pathway. Real Time-PCR to detect the main m RNA expression of PI3K/Akt pathway and APP. IHC method to detect the protein of PI3K/Akt pathway in mice’s hippocampus. Electron microscope to observe the apoptosis in mice’s hippocampal CA1 region. Result1.Morris water maze test showed that compared with the control group, Mice in model group’s platform latency, the first time reach for the original platform and total swimming distance increased significantly(P < 0.01), through platform number and the target quadrant time reduced significantly(P<0.01). Compared with the model group,each group’s platform latency and total swimming distance reduced(P<0.01,P<0.05), the target quadrant time increased(P<0.01,P<0.05). The high dose group and the equivalent does group’s through platform number increased(P<0.01,P<0.05), and presents a certain concentration-response relationship between two groups. Compared with Donepezil group, high dose group and the equivalent does group’s total swimming distance reduced significantly(P < 0.01). Compared with Changpuyizhiwan group, high dose group and the equivalent does group’s total swimming distance reduced significantly(P<0.01); high dose group’s through platform number increased(P<0.05).2. Diving platform test showed that compared with the control group, Mice in model group’s wrong number increased significantly(P<0.01), latency period reduced significantly(P<0.01). Compared with the model group,Donepezil group and high dose group’s wrong number reduced(P<0.01,P<0.05); Donepezil group and Changpuyizhiwan group’s latency period increased(P<0.01,P<0.05).3. Western Blot showed that compared with the control group, mice’s hippocampus in model group’s Akt and Bcl-2 relative transcript level reduced(P < 0.05). Compared with the model group, Donepezil group, Changpuyizhiwan group’s Akt relative transcript level increased(P<0.01,P<0.05); Changpuyizhiwan group’s Bax relative transcript level reduced(P<0.05); each group’s Bcl-2 relative transcript level increased(P<0.01,P<0.05).4. Real Time-PCR showed that compared with the control group, mice’s hippocampus in model group’s APP m RNA, PI3 K m RNA, Akt m RNA, Bcl-2 m RNA expression reduced, Bax m RNA expression increased. Compared with the model group, Donepezil group and the equivalent does group’s PI3 K m RNA, Akt m RNA, Bcl-2 m RNA expression increased, APP m RNA, Bax m RNA expression reduced; Changpuyizhiwan group’s APP m RNA, PI3 K m RNA, Akt m RNA, Bcl-2 m RNA, Bax m RNA expression reduced; high dose group’s APP m RNA, PI3 K m RNA, Akt m RNA, Bcl-2 m RNA expression increased, Bax m RNA expression reduced.5. IHC showed that the control group mice’s hippocampal CA1 region’s cells were complete, in alignment, normomorph, cell membrane and cytoplasm with deep brown granules. The model group’s cells were disordered arranged and shallow dyed. Compared with the model group, each group’s cells were in alignment deeply dyed.6. Electron microscope showed that the control group mice’s hippocampal CA1 region’s cells were normomorph, the nucleolus is obvious. Compared with the control group, each group is in apoptosis, and model group is the most serious. Compared with the Donepezil group, high dose group and the equivalent does group’s nucleus were lack of nucleoli, with vacuoles. Compared with the Changpuyizhiwan group, high dose group’s neuronal cytoplasm was shallower, nucleus were larger, chromatin gathered less and less vacuoles; equivalent does group’s nucleus were larger. Conclusion1.The Origin Qi is the basis of life activities of viscera and brain function. Full of Origin Qi lead to sober, lack of Origin Qi lead to insobriety and phlegm stagnation. Vitality Consolidation Treatment can increase Origin Qi, which is the important therapy for AD’s prevention and treatment.2. Vitality Consolidation Treatment can improve APP/PS1 double transgenic mice’s learning and memory ability.3.Vitality Consolidation Treatment can activate PI3K/Akt pathway and prevent cell apoptosis.