Expression Of Apoptosis Genes In Dastarcus Helophoroides

Dastarcus helophoroides Fairmaire(Coleoptera: Bothrideridae)known as the most valuable natural enemy insect against many large-body longhorned beetles.Under laboratory conditions,D.helophoroides can live for more than 8 years with continued sexual reproduction.Recent studies revealed apoptosis genes in D.helophoroides related to a process of programmed cell death that occurs in multicellular organisms.To date,no reports regarding to it issue were published.Thus,in this study,apoptosis genes from D.helophoroides were cloned,their mRNA expression was also detected.And the expression of apoptosis gene family in different tissues,different stages and different alive time adults were studied by using real-time quantitative PCR technology.The main results are as follows:1.Isolated a caspase-3 gene from D.helophoroides,in which caspase-3 has a full length of sequence with 1128 bp and contained 1008 bp open reading frame that encoded 335 amino acids.2.Identified a cenlular tumor antigen p53 gene from D.helophoroides.This gene has a full length of sequence with 1319 bp and contained 1035 bp open reading frame that encoded 344 amino acids.3.Differential expression of caspase-3 was significant differences among development stage,tissue,as well as,caspase-3 mRNA transcription levels were over-expression after high temperature treatment in both three development stages(adult,pupae,larvae).Based on the results of this research we suggest that caspase-3 gene may play an important defensive role in the apoptosis process of D.helophoroides under high temperature conditions.4.The result expression shown the cenlular tumor antigen p53 mRNA transcription levels gene expression levels were significant differences among development stage,tissue,as well as,this gene also was over-expression after high temperature treatment in both all development stages(adult,pupae,larvae).Increased expression of the cenlular tumor antigen p53 gene after high temperature treatments suggested that the gene could involved to control cell cycle progression in response to cellular stress like temperature-sensitive.