Methods And Experiment Research On Laser Sorting Cells In Microfluidic

Cell sorting in a variety of fields,such as the early disease diagnosis treatment and biochemistry,has great application prospect.Traditional cell sorting methods need a lot of samples,and need to tag on cells.They seriously affect the cell purity and activity.With the development of Micro Electro-mechanical System(MEMS),microfluidic chip technology obtaines the widespread attention.Because it has the advantages of save reagent,fast efficient and scale integration.Single mode fiber laser effects on cells in the form of the very mild and non-mechanical contact,achieving the effect of no damage cells.So in recent years laser sorting cells combines with microfluidic chip technology.Based on the study of microfluidic chips and the micro-scale laser field application at home and abroad,this paper contrasts and analyzes the current existing cell sorting methods.It hings with microfluidic technology and laser cell selection mechanism and experimental study,The paper puts forward a cell sorting method with no damage,no tags,no contact based on the optical radiation pressure.It builds cell screening separation model and sets up open the light flow control experiment platform.On this basis the paper completes distance experiment of laser acting on cells,which produces larger migration path in microfluidic.Cells efficient sorting is finished.The content mainly includes the following aspects:(1)Microfluidic cell offset mechanism research based on the optical radiation pressureBased on the study of the theory of the single mode fiber laser optical trap force,the particles distance mathematical model is established Under the comprehensive function of Stokes force and radiation pressure.by means of photon flow method the distance formula is derived in microfluidics.Optical radiation pressure acts on cells to produce displacement deviation.Influence law is revealed that is the relationship between cell migration distance with laser parameters,particle parameters in microfluidic channel.(2)The simulation experiment of laser sorting cells and influence parameters in microfluidicsThe cells optofluidic finite element simulation model is established based on single mode fiber laser optical.The Navier-Stokes equations,Helmholtz wave equation,and the Momentum theorem are coupled solution.Cell migration influence law is analyzed based on single factor of laser parameters(laser power,fiber core diameter),the microfluidic parameters(solution temperature)and cell parameter(cell diameter).The research provides theoretical basis for the cells efficient screening.(3)Developing half open sorting cells optofluidic chipOn the basis of microsize effect,cells are sorted in full open and full closed microfluidic chip,the flow drag force is too large in microfluidics.Through the comparison and analysis half open sorting cells optofluidic chip is developed.The microchannel width is effectively increased.The fluid drag force(stokes force)is reduced.The laser acts on cells to produce large offset distance.The result is finishing the efficient cells sorting.The influence parameters law is analyzed.Loading position is optimized of single mode fiber laser cells radiation,and migration performance is improved.(4)Microchannel laser sorting cells experiment researchBased on optofluidic sorting cells experiment platform,single mode fiber laser acts on yeast cells and polystyrene spheres.The two kinds of particles produce different offset distance so as to achieve screening in the perpendicular direction.Function mechanism is studied which is the relationship between laser power,microfluid velocity,solution temperature between cells offset distance in microfluidic.