Study Of Cell Ceparation Technology Drived By Laser Based On Microfluidic Chip

In biological and medical research, the separation of different types of cells has been an important field in cell research, which can bring great convenience to the diagnosis and treatment of many diseases and a breakthrough in life science research. Microfluidic lab on a chip is a new technology with wide concern, which realizes liquid manipulation in the micro/nano scale and can be used to study the cellular and molecular biology, etc.. Laser micro manipulation technology based on the radiation pressure and optical tweezers theory realizes the manipulation of cells, which has the advantages of high efficiency, no contact,no damage. Combining microfluidic and laser technology to realize high-efficiency cells separation has important theoretical significance and practical value.This paper mainly studies the cell separation based on the combination of laser driving and microfluidic chip. Through analyzing the present situation of domestic and foreign and doing a large number of literature review, the paper firstly studies the structure design and fabrication of polydimethylsiloxane(PDMS) microfluidic chip, and then explores the behavior of the fluid and the cells in the chip. At the same time, explore the cell driven by focused laser micro beam from theory and experiment. Finally the integrated cell separation system is designed and built based on microfluidic technology and laser driving method.First of all, this paper explores the methods of processing microfluidic chip and make an optimization. Choose the PDMS as the chip material, design process of chip based on soft lithography technology and vacuum plasma bonding, and finally realize the microfluidic chip fabrication. In addition study the behavior of micro fluid and make the simulation experiment of forming single cell flow by hydrodynamic focusing through using the COMSOL software. Then a system based on processing chip for hydrodynamic focusing is set up to do lots of experiments,which can confirm the simulation results.Secondly, this paper study the development and the principle of optical tweezers,which is analyzed from the aspects of ray optics regime, Rayleigh regime, etc.. Then make a force analysis of the particle captured and driven by laser through optical fiber. In addition, the experimental platform is built to do experiments and summary, which achieves the ultimate success of driving the yeast cells by the focused laser through optical fiber.Based on the above research, a system of cell separation driven by laser based on microfluidic chip is designed in this paper, which includes microfluidic chip as the centerand the comprehensive application of hydrodynamic focusing, the laser driving and a variety of detection methods. It has the innovation and feasibility. Because of the problem of time, technology and related equipment, there are still many problems in the system and need a further exploration and improvement.