The Application Of Biomimetic Substrate In The Detection Of SERS And The Process Of Targeted Drug Release

Biological materials are ubiquitous in our life.Cells can grow and die.Plants can grow constantly by accumulating nutrients through photosynthesis.Eyes can catch light and see objects.Animals can run and fly.They all belong to the category of biology and biological materials.We know that all living things on the earth are formed by the combination of inorganic or organic materials,such as our beating heart day and night,the tall trees in the forest,and the colorful corals on the seabed.From the view of Material Chemistry,it is an inconceivable phenomenon that cells,fibers and organs formed by only a few polymer materials can perform so many functions.For this reason,human beings have never stopped learning from nature,and a variety of biological materials in nature also provide endless inspiration for human manufacturing materials and tools.The research content of Biomimetic Materials Science is to clarify the material structure and formation process of organisms,to think about artificial materials from the perspective of biological materials,and to consider the design and production of materials from the perspective of biological functions.Bionic superhydrophobic materials and biomimetic drug carriers are functional materials developed under the guidance of biomimetic ideas.Bionic superhydrophobic materials are a kind of functional materials with superhydrophobic properties,which are prepared by mimicking the structure and physical and chemical properties of superhydrophobic materials in nature.They are widely used in anti-corrosion,anti-frost protection,self-cleaning and other fields because of their special micro-nano structure and super hydrophobic properties.Recent studies have shown that superhydrophobic materials can also be used in the detection of Surface Enhanced Raman Scattering(SERS).Hydrophobic interaction of superhydrophobic surfaces can enrich the samples to be detected,which greatly reduces the amount of substances to be detected in the process of analysis.In addition,nanoparticles with SERS-active surface provide a structural basis for the detection of SERS.As early as the beginning of 1980 s,researchers realized that synthetic nanoparticles could be used as carriers of some drugs for drug release.Since then,research on biomimetic drug carriers has never stopped.Drug delivery systems biomimetic has prepared a series of biomimetic drug carriers by surface modification of amino acids,sugars and lipids.Specifically,the shell structure,size and shape of cell-like drug carriers will play a decisive role in the application of drug carriers in cells.Therefore,biomimetic drug carriers obtained by surface functionalization of biomimetic nano-drug carriers using biotic ligands and materials similar to cell membrane structure can achieve targeted drug release in organisms.Biomimetic drug carriers are mainly divided into two categories: biological entity carriers and synthetic drug carriers.For example,inactivated viral carriers and bacterial carriers belong to biological entity carriers.In practical application,biomimetic nanoparticle drug carriers prepared by nano-engineering technology have higher safety.In this paper,two biomimetic substrate materials with SERS activity were prepared and applied to the analysis of SERS and targeted release process of anticancer drugs.The main contents are as follows: 1.Preparation and application of biomimetic superhydrophobic SERS substrateA superhydrophobic SERS substrate mimicking lotus leaf structure was prepared by chemical synthesis and surface modification.Polystyrene microspheres and silver nanoparticles with good SERS properties are constructed on glass substrates by self-assembly method to form micro-nano structures similar to lotus leaf morphology,and long-chain alkyl sulfhydryl molecules with low surface energy are modified on the surface to form detection substrates combining superhydrophobic and SERS-active.When the superhydrophobic SERS substrate is applied to the detection of SERS,the probe molecules to be detected can be effectively enriched and concentrated within nano scale of the substrate surface,which greatly improves the sensitivity of the detection of SERS.2.Quantitative analysis of glucose using superhydrophobic detection chipBased on the structure of superhydrophobic SERS substrate,a glucose detection chip is prepared by further modifying the substrate.The hydrophobicity of the substrate is used to enrich the droplets to be detected and reduce the amount of samples to be detected.At the same time,the quantitative analysis of glucose molecules can be achieved by monitoring the influence of enzymic catalytic reaction on the structure of molecular electron cloud.3.The sensitivity of the detection of SERS improved by the combination of SERS technology and molecular template technologyBiomimetic superhydrophobic SERS substrates are combined with molecular template technology and applied to the analysis of probe molecule.Molecule templates with the function of adjusting the orientation of probe molecules on the surface of substrates are prepared by modifying Monolayer film on the surface of superhydrophobic SERS substrates.Based on the surface selection rule of SERS,the sensitivity of probe molecules on the surface of substrates can be further improved by adjusting the molecular orientation of the target adsorbed on the surface of metal SERS substrates.4.Targeted release of anticancer drugs in living cellsBased on the idea of bionics,a mesoporous silica nanoparticles drug carrier is prepared by mimicking the structure and working principle of cell.The drug carrier has high drug loading efficiency,low cytotoxicity and good SERS-active.It can monitor the drug loading process in real time.At the same time,the p H response material on the surface of the carrier enables the drug carrier to achieve targeted release of drug molecules in target cells.