Research Of On-chip Peristaltic Micropump And Its Application

Micropump has become a new driving source for the microfluidic chip due to its advantages of miniaturization,large-scale integration,microamount working reagent,high ratio of reagents utilization,attracts many researchers'attention in the application of biochemical analysis.Peristaltic micropump as a type of multi-chambers mechanical micropump has been widely used in the applications such as drug delivery,cell culture,on-chip mixing and droplet generation,because the properties of the driving liquid are unlimted and compatible with high resolution,fast response time and large backup pressure.In this thesis,an on-chip peristaltic micropump technology has been developed to optimize the response time,dead volume and manufacturing process,as well as the cost and volume of working reagent.The low cost micropump is integrated on a plug-and-play microfluidic chip,which the actuators can be reused.Also the working principle,design of microfluidic chip,microfluidic system eastabishment and specific applications have been demonstared in the thesis,as follows:1.A novel technology of on-chip peristaltic micropump has been proposed with module design and simple structure of actuators and microfluidic chip.And a mathematical transportation model is established to analyze the relationship between the geometry of microfluidic chip and the driving parameters of actuator.The manufacture of micropump system is based on this mathematical transportation model,the processing and assembly of actuator array and microfluidic chip fabricating are researched in this thesis,as well as the characteristics of micropump transportation at different driving parameters and chip structure.The experiment have demonstrated that the high resolution(8.4nL/s),small dead volume(<300nL),transportation of bidirection,fast response time(?12.5ms),and microliter working reagent of the micropump,which shows great potential applications in biochemical analysis.2.Research of double channel peristaltic micropump mixer technology.To solve the problems of microreagent mixing inefficiently,a two-phase chip mixer bases on double-channel peristaltic micropump has been proposed,which can actively complete the mixing of microreagent with high efficiency.Utilizing the characteristic of micropump periodic pulsation at low driving frequency,a constant flow model is applied to mix the microreagent with a relative mixing index of 0.278.In addition,a pulsed working mode is applied to realize active mixing of microreagent with a relative mixing index of 0.06,which improves the mixing quality and efficiency.3.Research of droplet generation technology bases on peristaltic micropump.For the multi-chambers peristaltic micropump cannot effectively gnerate droplets,we propose a size-tunable droplet generation system bases on peristaltic micropump under the negative pressure,as well an on-demand droplet system.This droplet generation system may unfold new capacities of small volume application and low-cost size-tunable droplet reaction biomedical analysis.By changing the hydrodynamic resistance of channel by controlling the flow rate of micropump,the droplet length and formation frequency can be controlled actively under the negative pressure(81.5 ?m to 345.5?m).The main innovation of this thesis is the peristatic micropump including the separation design of actuators and microfluidic chip,using disposable chip which decreasing the volume of reagent and avoiding the cross-contamination.Also the mathematical transportation model provides theoretical reference for the microcahnnel geometry design.The applications of micromixing and droplet generation is explored in this thesis,which using a double channel peristaltic micropump mixer and a size-tunable droplet generation system.And the different working models of peristaltic micropump show great potential applications of Lab-on-a-chip.