Soil Microbiology Community Structure And Biological Monitoring Of Chinese Fir Plantation

Soil microbiology play an improtant role in soil formation,ecosystem balance and bioremediation.A reasonable microbiology genetic diversity is the key of maintaining soil productivity.Microbiology could reflect the change of soil fertility condition and an improtant indicative factor of soil fertility.The soil fertility of Chinese fir plantations have a large range of span due to different of management.So there is needed to establish a biological monitoring index.This study employed 454 pyrosequencing to analyze fifteen soil samples from the Chinese fir plantation forests at the Huangfengqiao farm.The fifteen soil samples were collected from the plot of first young Chinese fir(FYC),succession young Chinese fir(SYC),rotation young Chinese fir(TYC)and different developing stages of Chinese fir,respectively.This study aims to provide an overview of soil microbiology diversity and community composition in soils of Chinese fir plantations,examine the relationship between soil microbiology diversity and environmental factors,establish a biological monitoring index and supervise the soil fertility.The result will provide the theoretical basis of Chinese fir plantation soil fertility restoration.The main conclusions are as follow.(1)The soil physico-chemistry characteristics,enzymatic activity and undergrowth vegetation diversity of Chinese fir plantations.?Rotation different tree will help soil physico-chemistry characteristics and enzymatic activity to recover.By rotation,the available N,catalase activity,cellulose activity jumped higest,rising 36.68 mg/kg,0.81 mg/g,20.83?g/g,respectively.The undergrowth vegetation diversity shannon index declined 0.1.The soil physico-chemistry characteristics,enzymatic activity and undergrowth vegetation shannon index reduced by succession.The available N,catalase activity,cellulose activity and undergrowth vegetation shannon index reduce by42.46 mg/kg,1.23 mg/g,43.6 ?g/g,0.09,respectively.?The soil physico-chemistry characteristics,enzymatic activity and undergrowth vegetation diversity become better after ripe age.In the different forest age,the soil physico-chemistry characteristics,enzymatic activity and undergrowth vegetation diversity was young ages(FYC)>ripe ages(FRC)>middle ages(FMC).In the ripe age,the organic matter,urease activity and cellulose activity rised 26.86g/kg?2.35 mg/g?32.34?g/g,respectively.It can conclude that(2)The fungal diversity and community composition of Chinese fir plantations.?The fungal diversity and OTU numbers reduced by rotation.Shannon index declined 0.05,OTU numbers declined 283.The fungal diversity and OTU numbers reduced by succession.Shannon index declined 0.16,OTU numbers declined 201.The main different fungal between rotation and non-rotation place was Eurotiomycetes,Pezizomycetesycetes,the main different fungal between first young Chinese fir and succession young Chinese fir was Glomaceae.?In the different forest age,the soil fungal genetic diversity was FYC>FRC>FMC,the simpson index was 0.0249 at FMC.The OTU numbers declined with tree growing up,the OTU numbers of FYC,FMC and FRC was 1035,749,542,respectively.The relative abundence of Glomeromycota was reducing before the FRC period,then rising after the FRC period.? In Chinese fir plantation,the dominant fungal genus was Mycosphaerella,Hygrocybe,Magnusiomyces,Lipomyces.?The representational difference fungi was Eurotiomycetes,Glomaceae,Agaricomycetes,Pezizomycetesycetes.(3)The bacterial diversity and community composition of Chinese fir plantations.?The bacterial diversity and OTU numbers rised by rotation.The shannon index rised by 0.33 and the OTU numbers rised by 1179.The fungal diversity and OTU numbers reduced by succession.Shannon index declined 0.32,OTU numbers declined 769.The main different bacterial between rotation and non-rotation place was Bacteroidetes,Burkholderiales,Nitrosomonadales,Acidothermaceae,Micromonosporaceae,the main different bacterial between first young Chinese fir and succession young Chinese fir was Planctomycetes,Pseudomonadales.? In the different forest age,the soil bacterial genetic diversity and the OTU number was FYC>FMC>FRC.With the age going,the relative abundence of Burkholderiales,Xanthomonadales,Ktedonobacteria,Nitrosomonadales,Anaerolineae,Holophagae was diffrence.? In Chinese fir plantation,the dominant bacterial genus was Acidothermus,Bradyrhizobium,Lactococcus,Planctomyces,Sorangium,Bryobacter?The representational difference bacterial was Burkholderiales,Acidothermaceae,Acidobacteria,Nitrosomonadales,Xanthomonadales,Rhizobiales.(4)Biological monitoring of Chinese fir plantations.?The correlation of soil micro-diversity,and characteristic community with 14 enviromental factors were analyed by Spearman and RDA.The oil micro-diversity,and characteristic community was correlation with undergrowth vegetation,total N,pH and sucrase activity.? We establish a Chinese fir soil biological monitoring index,including Eurotiomycetes,Glomaceae,Agaricomycetes,Pezizomycetesycetes,Burkholderiales,Acidothermaceae,Acidobacteria,Nitrosomonadales,Xanthomonadales,Rhizobiales,pH,organic matter,total N,avaliable N,avaliable K,avaliable P,moisture content,vegetation diversity index,urease activity,catalase activity,Sucrase activity,Cellulose activity.We using the soil biological monitoring index monitored the soil fertility condition,the soil fertility ranking was FYC>TYC>FMC>FRC>SYC.