Study On The Protection Of Tanshinone ?A Sodium Sulfonate On Cardiac Function In Mice With Myocardial Infarction

Objective: Build a stable and reliable model of myocardial infarction(MI)in mice to lay a solid foundation for the research on the mechanism of drug treatment in mice with myocardial infarction.Methods: After fasting for 6-8 hours,the mice were intraperitoneally anesthetized with pentobarbital sodium.The mice were intubated and connected to a ventilator to maintain normal respiration.Then they were performed a left thoracotomy to expose the heart.MI was induced by surgical ligation of the LAD coronary artery by an 8-0 polypropylene suture passed about 2-3 mm from the inferior margin of left auricle.The successful induction of MI was confirmed based on the observation of the pale color of the anterior portion of the left ventricle and electrocar-diagram(ECG)ST-segment elevation.The thorax was closed by surgical sutures.Results: All the mice model were successfully created.Three weeks after myocardial infarction,all MI mice developed myocardial remodeling measured by left ventricular ejection fraction and some other indexes through echocardiography.Some mice died in different time periods after MI.Conclusion: Ligation of coronary artery is an accurate and feasible method to create model of myocardial infarction.It is a reliable and practical way to study the mechanism of drug treatment after MI by using myocardial infarction model.Objective To explore whether Sodium tanshinone ?A sulfonate(STS)could protect cardiac function after myocardial infarction(MI)and mechanism of the protective effectsMethods The MI model was created by ligating the left descending coronary artery.Then the mice were randomly divided into three group: sham group,STS group,untreated group.Three weeks after MI,recorded mortality of each group.Cardiac function were measured through echocardiography.Masson and HE staining were done after completing the pathological section.TUNEL,TNF-?,IL-1?,Bcl-2,Bax,VEGF,?-SMA,CD31 were detected by immunohistochemistry and Bcl-2,Bax,ASK,phospho-ASK,e NOS,phospho-e NOS,NF-?B were detected by Western Blot.The SOD,GSH and MDA were measured by biochemical detection.Results Compared with untreated group,the mortality of STS group was significantly decreased and the cardiac function of STS group significantly improved measured by LVEF and LVFS.Moreover,STS could reduce inflammatory cytokines,increase resistance to oxidative stress,resist myocardial apoptosis and promote angiogenesis.Further study on H9c2 cells showed the effects of anti-apoptosis.Conclusion Our results show that STS can improve cardiac function,reduce inflammation,resist apoptosis and promote angiogenesis after MI.