Enolase-Phosphatase 1 As A Novel Potential Malignant Glioma Indicator By Promotes Cell Proliferation And Migration

Backgroud and aims:Enolase-phosphatase 1(ENOPH1),an enzyme that is involved in polyamine biosynthesis and is associated with stress responses,but little is known about its role in the pathophysiology of glioma.In this study,we made an effort to explore ENOPH1 expression and function in human glioma tissues and cell lines.Methods:In this study,western blot,qPCR and immunohistochemistry analysis were performed to investigate the expression of ENOPH1 protein in glioma tissues in 74 patients.3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide(MTT)assay,wound healing,western blot,Immuncytochemical detection and cell cycle assay were carried out to determine cell growth and cell migration in U87 and U251 glioma cellsResults:It has been showed that the level of ENOPH1 was significantly increased in glioma tissues compared with normal brain tissues.In addition,the expression level of ENOPH1 was positively correlated with glioma pathological grade.Knockdown of ENOPH1 expression with siRNA not only significantly decreased cell proliferation,but also markedly inhibited cell migration.Interestingly,knockdown of ENOPH1 promoted its downstream protein aci-reductone dioxygenase 1(ADI1)translocation from the nucleus to the cytoplasm in glioma cell,while MT1-MMP expression was significantly downregulated compared with the control group.Conclusion:Taken together,it is demonstrated by our data that knockdown of ENOPH1 suppressed glioma cells growth and migration,which may be associated with ADI I translocation and MT1-MMP downregulation in glioma cells,thus ENOPH1 could serve as a new potential therapeutical target of glioma.