MiR1976/CD105/integrin ?v?6 Pathway In Escherichia Coli And Staphylococcus Aureus Study On The Role And Mechanism Of Infection-induced Vaginitis

The incidence of vaginitis is very common among women worldwide,especially women of childbearing age,and it is associated with a significant increase in preterm birth and pelvic risk in inflammatory diseases.The imbalance of vaginal flora is the main cause of vaginitis,and ultimately promotes the occurrence and development of vaginal infection.However,the relevant pathogenesis remains poorly understood.pelvic inflammatory diseases and an imbalance in the vaginal flora.Objective : The role of miR1976/CD105/integrin ?v?6 pathway in vaginitis caused by Escherichia coli and Staphylococcus aureus infection along with understanding of its possible mechanism.Methods:Using a mouse vaginitis model infected with Escherichia coli and Staphylococcus aureus,a variety of molecular biological methods were employed to study the expression and correlation between microRNA1976,transforming growth factor receptor CD105,integrin ?v?6 as well as to confirm whether the signaling pathways were associated with vaginal infections.Results: We demonstrated for the first time that the microRNA1976/CD105/integrin ?v?6 axis regulates Escherichia coli or Staphylococcus aureus-mediated vaginal infection in mice,as evidenced by the finding that Escherichia coli or Staphylococcus aureus-induced vaginal infection was reversed in mice by microRNA1976 overexpression and exacerbated by CD105 overexpression.The regulation of CD105 and integrin ?v?6 by microRNA1976 was further confirmed in amurine model of vaginitis with adenoviral vector treatment.Conclusion:Taken together,our data suggested that microRNA1976 negatively regulates Escherichia coli or Staphylococcus aureus-induced vaginal infection in mice at least in part by suppressing CD105 and integrin ?v?6 expression.These findings may provide new insight into the molecular mechanisms of Escherichia coli or Staphylococcus aureus-induced vaginal infection,identify a novel diagnostic and prognostic biomarker for flora imbalance-associated vaginitis and offer a potential therapeutic target for vaginitis.