| Parkinson's disease is a chronic degenerative disease influencing the central nervous system,which mainly affects the motor and non-motor nervous system functions of patients.At present,there is not any complete cure method for PD.Drugs combined with surgery,rehabilitation,psychological,nursing,and other cross-disciplinary integrated treatment can alleviate some symptoms of PD,but these treatments cannot completely eliminate the symptoms of patients,and long-term use of drugs often has varying degrees of side effects.Transcranial direct current stimulation(t DCS)is a kind of non-invasive brain stimulation(NIBS)developed in recent years to regulate the activity of cerebral cortical neurons.Previous studies have concluded that it can cause biphasic,polardependent changes in the cerebral cortex can cause sustained cortical excitation or inhibition and may alleviate the motor and non-motor symptoms of Parkinson's disease.However,the therapeutic mechanism of this model is either still in the exploratory stage.In this study,before the application of t DCS in the clinical treatment of Parkinson's disease,the animal model of Parkinson's disease was used and a series of experimental methods were designed to verify the possible therapeutic potential of t DCS for Parkinson's disease and the mechanism of neural action.Objective: To research the neuroprotective mechanism in Parkinson's disease model induced by 6-OHDA treated by t DCS.Methods: Sixteen male PD rats induced by 6-OHDA were randomly divided into t DCS group(n=8)and non-t DCS group(n=8).We observed the change of Body weight,gait,rotation test,open field test,short-term memory ability,depression-like behavior and anxiety-like behavior and brain histology.The recorded values were statistically analyzed.Results: We observed that there was no significant differences in body weight and Rotation experiment between the two groups.And we found that in the progress of time,the two sets of gait parameters have increased.Compared with non-t DCS group,there were significant differences in gait parameters t DCS group.Through the Bar test and open field experiment,we found that t DCS can delay the forefoot locomotor function and autonomic motor ability of PD rats.However,there was no significant difference in shortterm memory ability between the two groups.While depression and anxiety-like behavior of rats in the t DCS treatment group was alleviated.Compared with the non-t DCS treatment group,the number and density of TH+cells and fibers in the substantial nigra and striatum of the t DCS's treatment group were increased.Conclusion: We analyzed the detailed spatiotemporal changes of gait pattern to investigate the potential benefits after four-week t DCS treatment in the rat model of PD,and the t DCS could improve the motor and non-motor function of the defect,increase the number of TH + cells and fibers in substantial nigra and striatum of Parkinson's disease rates,which provides a preliminary experimental basis for further study of the neuroprotective mechanism of t DCS. |
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