| PURPOSE:To study the effects of ketamine on dopaminergic neurons in Parkinsonian rats models,and then to explore different anaesthesia methods on PD's rat models.Methods:The rats models of PD were made by injecting stereotaxically 6-OHDA in ketamine- and isoflurane-induced anaesthesias.There were four groups and the rotational behavior was induced by apomorphine. At the end of the experiment all rats were made internal fixation,then TH staining were made. In this study we observed the rotational behavior and the injury of substantia nigral cells on ethology and pathology.Results: There were three sections in this experiment.The first section was to take practical training and tests to all the rats; The second was to make Parkinsonian rats models,and its achievement ratio was 100﹪. The third section was to repeat practical tests on the Parkinsonian rats models. Also there were two phases in the second section. The data showed difference on the rotational behavior between the first and the second group (P<0.05).The sham operation group (the third group) and the controlled group (the fourth group) didn't show any freatures. The numbers of the TH+ neurons in the left SNc of successful Parkinsonian rats models in the first and the second group were less than that of the third and the fourth group obviously (P<0.05). And concerning it,the data showed difference between the first and the second group (P<0.05),but no difference between the third and the fourth group. With regard to the number of the TH+ neurons in the left SNc, there were obvious difference among the four groups.Conclusion 1. It is reliable to inject 6-OHDA to the left SNc to create rat model of Parkinson's disease according the target site 2. Ketamine could influence the rotational behavior of Parkinsonian rats models and the remaining dopamine neurons'appearance and function; 3.The new practical tests like Stepping test, Postural Balance test can be used to identify the lesion effect in rat models of PD. |
PDF Full Text Request