| Lipid peroxidation in vivo was investigated by measuring lipophilic non-polar and polar aldehydes and related carbonyl compounds, the secondary degradation products of lipid peroxidation, in rats and human subjects under normal or oxidatively stressed conditions. A variety of 2,4-dinitrophenylhydrazine derivatives of these urinary compounds were separated and identified by a newly developed high performance liquid chromatographic method.;Response of urinary lipophilic aldehydes and related carbonyl compounds to dietary and chemical factors that stimulate lipid peroxidation in vivo was measured. Most of the compounds were increased in rats due to the pro-oxidant effects of vitamin E deficiency, high polyunsaturated fatty acid administration, and CCl;Urinary levels of lipophilic aldehydes and carbonyl compounds were measured in streptozotocin-treated diabetic rats. Significant increases of butanal butan-2-one, pentan-2-one, hexanal, hept-2-enal, octanal, non-2-enal, deca-2,4-dienal, 4-hydroxyhex-2-enal, 4-hydroxynon-2-enal, and several unidentified carbonyl compounds were found in diabetic rats as compared to the normal animals. Enhanced excretion of lipophilic aldehydes and related carbonyl compounds in diabetic rats confirmed increased levels of lipid peroxidation in vivo due to streptozotocin treatment. After the onset of diabetes, the antioxidant treatment with vitamin E (RRR-tocopheryl acetate) or probucol resulted in significantly decreased excretion of some, but not all urinary lipophilic compounds.;Physiological levels of urinary lipophilic aldehydes and related carbonyl compounds were measured in normal rats and human subjects. A number of urinary compounds were identified by cochromatography in three different solvent systems using pure standards. These compounds were butanal, butan-2-one, pentan-2-one, hex-2-enal, hexanal, hepta-2,4-dienal, hept-2-enal, octanal, non-2-enal, deca-2,4-dienal, 4-hydroxyhex-2-enal, and 4-hydroxynon-2-enal. |
