The role of lysyl residues in the binding of FD gene 5 protein to nucleic acids

he structure and DNA binding function of fd gene 5 protein were studied using a combination of chemical modification experiments and nuclear magnetic resonance spectroscopy. The lysyl residues of fd gene 5 protein were reductively methylated to form N;An experimental protocol was designed to measure the reactivity of the gene 5 protein lysyl residues while the protein was bound to a nucleic acid polymer (i.e. a "protection" experiment). The experiment allowed the determination of the changes in reactivity of individual lysyl residues caused by nucleic acid binding. In addition, the experiment was used to introduce the ;Three of the protein lysyl residues (Lys24, Lys46, and Lys69) were strongly protected from methylation when the protein was bound to polyribouridylic acid. The resonances of these same three residues were uniquely perturbed when the protein bound a DNA oligomer, d(pA);The ;The...