The Role And Mechanism Of Diosmetin In Fat Deposition

Fat deposition is an important economic trait in livestock breeding,and as livestock with high-fat deposition capacity,the proportion of fat distribution in the body is a key factor in determining the quality of pork.At present,reducing the deposition of subcutaneous and visceral fat in pigs and increasing the deposition of intramuscular fat are effective strategies to improve meat quality.The precise regulation of fat deposition capacity by natural products is an efficient and safe method.Diosmetin,a natural compound with a wide range of sources,has been shown to have a similar chemical structure and presumably similar biological function to quercetin,which has been shown to significantly reduce fat deposition.It is suggested that Diosmetin has the potential to regulate fat deposition,but its regulatory role and mechanism need to be further investigated.Therefore,on the one hand,we used Diosmetin to treat mice and adipocytes at in vivo and in vitro levels to reveal the molecular mechanisms by which Diosmetin regulates fat deposition and energy metabolism in mice.On the other hand,because Diosmetin is safe to take and widely available,it can be used for post-feeding of pigs,thereby clarifying the effects of Diosmetin on fat deposition and meat quality.The following results were obtained through systematic studies.1.The diosmetin treatment prevented high-fat diet-induced obesity as well as genetic obesity.Diosmetin can prevent obesity in both high-fat diet-induced obesity mice and ob/ob mice;The histomorphological results showed that the area of adipocytes in the inguinal white adipose tissue and epididymal white adipose tissue of Diosmetin-treated mice was smaller than that in the control group;Diosmetin treatment reduced the expression of genes related to adipogenesis.2.Diosmetin treatment improved insulin resistance and glucose tolerance induced by a high-fat diet as well as genetic obesity.Diosmetin improved insulin resistance and glucose tolerance in high-fat diet-induced obese mice.Diosmetin treatment also improved insulin resistance and glucose tolerance in genetically obese mice.3.Diosmetin treatment prevents HFD-induced systemic inflammation and non-alcoholic fatty liver.Diosmetin alleviates high-fat diet-induced systemic inflammation and inflammation of adipose tissue.Diosmetin can prevent non-alcoholic fatty liver induced by a high-fat diet.4.Diosmetin treatment promotes thermogenesis in high-fat diet-induced obese mice.Diosmetin promotes thermogenesis in high-fat diet-induced obese mice.Diosmetin promotes the activation of brown adipose tissue in high-fat diet-induced obese mice.Diosmetin promotes the browning of white adipose tissue in high-fat diet-induced obese mice.5.Diosmetin regulates lipid deposition in fat cells by activating estrogen receptors(ERs).Diosmetin can effectively inhibit the adipogenic differentiation of adipocytes;Diosmetin inhibits adipogenic differentiation of adipocytes by activating estrogen receptorα(ERα)and estrogen receptor β(ERβ);Diosmetin promotes the expression of thermogenesis-related genes in adipocytes by activating ERα and ERβ.6.Diosmetin improves lipid metabolic function in mice by activating ERs.Diosmetin prevents insulin resistance and glucose tolerance induced by high-fat diet through the activation of ERs;Diosmetin promotes thermogenesis of brown adipose tissue in high-fat diet induced mice by activating ERs;Diosmetin promotes the browning of white adipose tissue in high-fat diet induced mice through the activation of ERs.7.Diosmetin supplementation in feed improved pig carcass traits and meat quality.Diosmetin addition did not affect the growth performance of fattening pigs,but reduced carcass fat content and backfat thickness;the Longissimus dorsi muscle tethering strength and p H were significantly higher in Diosmetin-treated fattening pigs than in the control group.Diosmetin supplementation in feed significantly increased the content of docosahexaenoic acid(DHA)and fresh amino acid in pork.In summary,this study clarified the role of Diosmetin in fat deposition in mice at both in vitro and in vivo levels,and revealed that Diosmetin increases energy expenditure and protects mice from high-fat diet-induced/genetic obesity and metabolic dysfunction by activating estrogen receptors.Furthermore,by adding Diosmetin to the diet to feed fattening pigs,it was found that the supplementation of Diosmetin to the feed can significantly improve the quality of pork.It provides a theoretical basis and scientific basis for Diosmetin derived from natural products to improve metabolic diseases and improve meat quality.