| Hypothalamic-pituitary-ovary axis(HPOaxis) is very important to the regulation mechanism of reproductionendocrine and have an close relationship with neuro-endocrine- immunoregulatory network; nenneurotransmitter, hormone and cytokine what exist in animal's body play an key role in HPO axis rely on them-self receptor. And them expression could vary in oder to maintin the balance of neuroendocrine regulation mechanism in different condition of immunity and physiology. In order to research the mechanism of different immunity affecting the neuroendocrine and the expression variation of hormone and cytokine at receptor level in different immunity. We select sensitive immunohistochemical MaxVision method to study the co-expression of interferon-γreceptor (IFN-γR)and estrogen receptor(ER) in in different immunity effect. The major results were as follow:1. In thirty-four nucleuses in hypothalamus of rats, includ the nucleus pre-opticus medialis, area anterior hypothalamic, nucleus ventromedialis, nucleus anterior commissuris, pararentricular nucleus, supraoptic nucleus, nucleus mamillaris medialis, et al, the IFN-γR, ER single or double labeled immunoactive substance were located in this nucleuses. And some glial cells co-exist with neuron. The IFN-γR immunoactiv substance located in the cytoplasm, the ER immunoactiv substance located in the cells nucleus or whole cell; the intensity of color of these immunoactiv substance some deep and some week.2. In the seventeen nucleuses in the hypothalamic of the immunity enhancement rats and immunity depressing rtas, the variation that the number of IFN-γR, ER single or double labeled immunoactive cells were very different and the variation were positive correlation with variation of immunity. These nucleuses includ pre-opticus periventriculars nucleus, pre-opticus suprachiasmatic nucleus and pre-optics medial nucleus.3. There were the IFN-γR, ER single or double labeled immunoactive cells in acidophilic cells, basophilic cells and unlike color cells in hypophysis of rat. The variation of expression in hypophysis similar to the variation in hypothalamus, that is to say, the variation of expression quantity were positive correlation with variation of immunity. There were the IFN-γR, ER single or double labeled immunoactive cells in neurohypophysis and neurofibers.4. In the corpus luteum, ovarian follicle granulose cell layers and ovarian stroma, there were a lot of IFN-γR, ER single or double labeled immunoactive cells expression.from wall to cabin at the ovarian follicle, the color of IFN-γR immunoactive substance were deeping increasingly, specially, the color of oocyte were most deep, and the color of corpus luteum were deeply. We speculate IFN-γrely on them-self receptor to affect the hormone production and releasing of ovary, growth and be matured of oocyte and regulation the function of corpus luteum. minewhile, we find the number of ER immunoactive cells in immunity enhancement rats more than control rats,but the number of IFN-γR/ ER duble labeled immunoactive cells in immunity enhancement rats not moer than control rats; the number of IFN-γR single labeled,ER single labeled and IFN-γR / ER duble labeled immunoactive cells all not the control rats.According to the research results, we believe IFN-γand estrogen play an key role in neuroendocrine regulation mechanism of HPOaxis rely on them-self receptor. And different immunity affect the production, releasing and coordination of hormone and cytokine; we can speculate that IFN-γrely on them-self receptor to stimulate estrogen production and releasing in rat hypothalamus, and the relationship of effect to estrogen was positive correlation.
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