The Study Of The Efects And The Mechanism On The Biological Behavior Of The VX2 Pyriformsinuscarcinoma Model With Qing Liu Liang Hou Fang

Objective:To Study of the effects on the biological behavior of the VX2 pyriformsinuscarcinoma(PSC) model with Qing Liu Ling Hou prescription; and to study the mechanism of the therapeutic effect with it.Methods:1. Establishment of Rabbit VX2 pyriformsinuscarcinoma model:To establish a rabbit VX2 hypolaryngocarcinom (HPC) model and observe it's growth. After anesthesia,tumor tissues from the VX2 cancer New Zealand rabbit was implanted to the rabbits'groin to passage.Then tumor tissues from the passaged- rabbits,was made tumor tissue mass suspension, and the suspension was injected into rabbit's pyriform sinus guided by rigid bronchoscope,2. The study of the efects on the biological behavior of the VX2 HPC model with Qing Liu Ling Hou prescription:46 rabbits of VX2 PSC model were divided into three groups in random:blank groups,cntrol groups of 5-F(FLUOROURACIL) and Chinese heb groups (Qing Liu Ling Hou prescription).7of the total 39 rabbits in the study died by accident. Therefore,the growth and metastatic character were observed by anatomy,CT and pathology. Immunohistochemistry staining by specific monoclonal anti-p27/Kipl protein,anti-cyclin D1 protein, anti-cyclin B protein and anti-CDK4 protein was performed using the S-P method..Results:1. The control groups and treatment groups in the living quality and duration were marked different than the blank groups.2. The control groups and treatment groups in anatomy and intervertebral were differently expressed as compared with those in blank groups. The control groups and treatment groups were significantly different with the blank groups in pathology. In immunology,the control groups were were significantly different with treatment groups, and the blank groups were significantly different with the control groups.Conclusion:1.To Qing Liu Ling Hou prescription; it can effectively improve the living quality, prolong the lifespan of the cancer rabbit.2. Qing Liu Ling Hou prescription can promote tumor-cells apoptosis in the VX2 PSC model by interventing expression of tumor-cells potentiality.3.The therapeutic principle of Qing Liu Ling Hou prescription may be based on promoting or inhibiting the expression of cellular potentiality,so it embodied the adjustion of the traditional chinese medicine on the body's immune function.