Research On Pharmacognosy And Chemical Constituets Study Of Cynanchum Chinense R.Br.

Object:In order to establish the quality standard of Cynanchum chinense R.Br., a systematic pharmacognostic research and isolation of chemical constituents of Cynanchum chinense R.Br. were performed.Methods:Pharmacognostic researches aimed at the botanical resource, medical character and microstructure of Cynanchum chinense R.Br. Scanning electron-microscope was applied in the micro-morphological research on its aerial part. Three-Steps Infrared Macro-Fingerprint Method was used to study the infrared fingerprint of Cynanchum chinense R.Br. from different area, of different parts, and of abstraction with different polarity solvent. Spectrophotometry was used to determine the content of total flavone. Its fingerprint was compared by applying High-Performance Liquid Chromatography (HPLC) to determine the concentration of kaempferitrin. Its chemical components of alcohol extractive were analyzed using systematic separation process. The test on its purity and the content of extract was performed using the methods from Chinese Pharmacopoeia.Results:This research determined the traits in the origin, character, and micro-morphology of Cynanchum chinense R.Br., and also determined the fingerprint of the infrared and HPLC. Besides, this research established the content standard of water, ash, extract, flavone and kaempferitrin.6 compounds were identified from Cynanchum chinense R.Br. and 20R-n-Butylpregn-5-en-3β-ol was isolated from botanical resource for the first time.4 compounds, daucosterol, lupeol, hexadecanoic acid glyceride and octacosanol, were isolated from Cynanchum chinense R.Br. for the first time and identified. The content of different inorganic elements and amino acid were analyzed. The standard for crude drug quality of Cynanchum chinense R.Br. was proposed initially. Conclusions:This research summarized systemic scientific data, and established the foundation for the pharmacognosy, quality control, quality standard, isolation and identification of chemical components, pharmacological activity of Cynanchum chinense R.Br., which is definitely helpful for further research on Cynanchum chinense R.Br.