Aboveground Biomass Of Active Ingredients Of Bupleurum Chinense

In this paper, in order to obtain the chemical constituents from Bupleurum chinense DC. The boiling water extract of Bupleurum chinense DC. was studied. Eleven compounds were isolated and purified by various chromatographic methods, such as macroporous resin column chromatography, silica gel column chromatography, sephadex LH-20 column chromatography and so on, and their structures were established by chromatographies and spectral analysis. They were elucidated as following: bupleurin(Ch-l), isorhamnetin (Ch-2), isorhamnetin-7-O-α-L-rhamnopyra-noside (Ch-3), kaempferol (Ch-4), kaempferol-3-O-α-L-arabinofuranoside (Ch-5), kaempferol-7-O-α-L-rhamnopyranoside (Ch-6),kaempferol-3,7-di-O-α-L-rhamnopyran-oside (Ch-7), rutin (Ch-8), 7-hydroxy-2,5-dimethyl-chromnone (Ch-9), β-sitosterol (Ch-10). Among them, compound Ch-1 is a new compound; compound Ch-5 and compound Ch-9 were isolated from the genus for the first time.The bioactivited contents of kaempferol and kaempferol-7-O-α-L-rhamnoside were determined by HPLC at the same time. And it leads to the possibility of the quality control.Bioactivity experiment indicated that the compounds from Bupleurum chinense DC. significantly increased the concentration of NO₂^- in the medium. And Ch-2(1 ug/ml), Ch-3 (1 wg/ml), Ch-7 (1 wg/ml) can enhance the induction of NO in macrophages by LPS. All the extracts can not influence the cell survival except for Ch-6 (10 wg/ml).