A Study On The Dynamic Changes Of Inflammation And Heat-sensitive Moxibustion Intervention Result After Plyometric Induced Muscle Damage

Objective: Use an acute Plyometric（PLYO）to induce Exercise-induced muscle damage（EIMD）,and verify the effectiveness of the modeling and the characteristics of PLYO-induced EIMD;to analyze the changes of plasma inflammatory factors,free radicals,antioxidant enzymes and the dynamic changes of plasma miR-133 a,miR-146 a,miR-21’s expression level after EIMD,and to explore the biological significance of plasma miRNA during the occurrence of EIMD.At the same time,do the intervention treatment with heat-sensitive moxibustion,and observe the effect of heat-sensitive moxibustion on EIMD and analyze the mechanism of action,and study its rationality and feasibility as an effective means of prevention and treatment.Through this study,it provides theoretical reference for scientific PLYO training,effective intervention EIMD and national fitness,and also provides new ideas for the research and practical application of heat-sensitive moxibustion in the field of sports rehabilitation.Methods: The subjects included 66 healthy male college students,and were simply randomly divided into blank control group（C group,n=22）,exercise group（E group,n=22）,heat-sensitive moxibustion group（I group,n=22）.C group live and study as usual,E group conducted an acute PLYO to induce EIMD,I group use thermosensitive moxibustion intervention to treat one acute PLYO-induced EIMD by bilateral Zusanli acupoints,40 minutes/ time,a total of 10 times.ISOMED2000 isokinetic muscle force system was used to determine the isokinetic muscle strength of knee extensor;plasma IL-6,IL-10,TNF-α,MCP-1,hs-CRP level was detected by ELISA;plasma CK,LDH,MDA,NO,GSH-Px,CAT,T-AOC content was determined by biochemical analysis method;plasma expression level was determined by real-time quantitative polymerase chain reaction（qRT-PCR）.Experimental data were analyzed by repeated measurement variance analysis and Pearson correlation analysis.Results:（1）After PLYO,the levels of VAS,plasma CK and LDH significantly increased,reaching the maximum at 24 h,48 h and 24 h after exercise.The PT of the knee extensor decreased obviously,and the recovery process showed double-peak change.The plasma levels of IL-6,IL-10,MCP-1,TNF-α and hs-CRP increased significantly after PLYO,reaching the maximum of 2 h,2 h,24 h,24 h and 24 h after exercise respectively.The values of plasma MDA,GSH-Px,T-AOC,CAT reached their maximum at 24 h,24h,24 h and 48 h after PLYO respectively;（2）The plasma miR-133 a,miR-146 a increased significantly at 2h after PLYO and recovered at 24 h.The changes of plasma miR-21 were not statistically significant.The modified variables of miR-133 a and IL-6（P= 0.044,r= 0.433）,TNF-α（P= 0.020,r= 0.491）were positively correlated.The modified variables of miR-146 a and IL-6（P= 0.019,r=-0.495）,TNF-α（P= 0.036,r=-0.450）,MCP-1（P= 0.010,r =-0.539）were negatively correlated.The modified variables of miR-133 a,-146 a were not correlated with the changes of CK and oxidative stress factors.（3）Comparing with E group,VAS,CK,LDH,IL-6,MCP-1,TNF-α,hs-CRP,MDA were significantly lower;IL-10,NO,T-AOC,GSH-Px,CAT was significantly higher after treatment of heat-sensitive moxibustion.Conclusion:（1）PLYO can effectively lead to EIMD.The obvious changes of plasma LDH,CK,inflammatory factors（IL-6,IL-10,TNF-α,MCP-1,hs-CRP）,oxidative stress（MDA,T-AOC,GSH-Px,CAT）can reflect the occurrence of EIMD.（2）Plasma miR-133 a,miR-146 a may be a product of stress in the course of EIMD caused by PLYO,or it may be the signal molecule for the transformation of early mechanical injury to metabolic damage in EIMD.（3）Heat-sensitive moxibustion at Zusanli acupoints has a significant relief effect on EIMD and the reduction of muscle strength caused by PLYO,and can effectively promote the recovery of EIMD through anti-inflammatory and antioxidant stress.