Mushroom Body Structure And Learning Memory Ability Of Olfactory As A Result Of Hydroxyurea Treatment On The Gryllus Bimaculatus

Objective:This study tries to identify mushroom bodies play a very important role in learning and memory by comparing morphological changes and behavioral brain changes in Gryllus bimaculatus which have been treated with Hydroxyurea to those of control group.Methods:Conventional paraffin sectioning and hemotoxylin and eosin (HE) staining were adopted to compare the morphological changes of brain in Gryllus bimaculatus between control group and treatment group. In addition, the behavioral research on the associational memory of olfactory sensation was employed to compare the behavioral changes between control group and treatment group.Results:The Cerebral ganglion in Gryllus bimaculatus is symmetrical from left to right, it include protocerebrum, deutocerebrum and tritocerebrumo The protocerebrum was composed by mushroom bodies, central complex and optic lobes. A Cerebral ganglion have two mushroom bodies, and the mushroom bodies is symmetrical from left to right.Central complex occupied central of the protocerebrum.Optic lobes amonged both sides of protocerebrum.externally ocular.The mushroom bodies composed by calyx and root.There are many Kenyon cell on the top of the calyx.The root of the mushroom bodies divided peduncle, a-lobe andβ-lobe.The central complex was constituted by central body and protocerebral bridge.The Gryllus bimaculatus who was feeded hydroxyurea, whose mushroom bodies changed very much, the mushroom bodies almost lost, the calyx disappeared, the quantity of Kenyon cell was reduced.There is a little peduncle remained in root, anda-lobe andβ-lobe was disappeared.The other part of the cerebral ganglion was no change.After training the group were not feded Hydroxyurea, whose PPI from before PPI=39.70 becomes,2h PPI=61.02,2d PPI=55.45 and 4d PPI=52.17.Used SPSS13.0, we find thai it changed significant. After training the group were feded Hydroxyurea, whose PPI from before PPI=39.34 becomes,2h PPI=40.71,2d PPI=39.26and 4d PPI=40.42.Used SPSS13.0, we find thai it changed not significant. Before training comparison the fed and not fed grops we found that it was no change. Conclusion:Behavior experiment illustration that:Whether fed Hydroxyurea or not to Gryllus bimaculatus can not effect it select odor before training, but that can effect it select odor after training.With anatomical results:hydroxyurea can damaged the mushroom bodies.lt explained that mushroom bodies play an important role.The damage of the mushroom bodies can lead Gryllus bimaculatus lost learning and memory ability about olfactory, but it can not affect its olfactory.