Research On The Structural Optimizaton Of Bioreactor With Elephant Ear Impeller And Study On The Shear Properties Of Cell

With the continuous transformation of biotechnology from laboratory research to practical and industrial, cell culture technology which is the important means of it has become a rushed field of study in many countries. Currently cell culture studies does not just stop at the laboratory scale, but develops towards the large-scale industrial-level training both at home and abroad, therefore, the bioreactor which is the core device of cell culture technology is required higher. In this paper, based on the analysis of affected factors of large-scale cultivation of cells, we found the main reason for restricting cell mass culture, which is mixed flow reactor uniformity and mixing shear force, therefore, making the two factors as research directions, we carried out a series of simulation calculations and provide information and theoretical basis for the research and design of the large-scale cell culture bioreactor.Firstly, using CFD simulation to analyze a new type of Elephant Ear impeller, and make a simulation comparison on it and the three commonly used impeller in the mixed flow uniformity and shear force, as a result, it founds that it not only produces less shear force but also produces more uniform flow mixing field, therefore, the impeller was used for the bioreactor.On this basis, using the simulation software FLUENT to optimize the structure of 100L bioreactor which is use Elephant Ear impeller, optimization of the reactor diameter ratio, bottom of the tank radius, impeller mounting height and the number of baffles; simultaneously, Elephant Ear impeller is analyzed too.Secondly, using the finite element analysis software ABAQUS simulation analysis the mechanical properties of CHO cells in the flow field from a broad perspective, as a result, obtained the law of cells stress and deformation by Shear force; Analysis of the mechanical properties of cells which is in the medium pressure and shear stress, and get the conclusion that the shear stress is the main reason for deformation and damage of cell.Finally, we combine the CHO cells cultured experiment under different speed test and simulation analysis, get the result that the most suitable shear stress for the growth of CHO cells in the shear flow field is 0.3Pa, simultaneously, simulate the effect of the shear stress on cells and get the result that the maximum deformation of cell in the U direction is 8.5μm.