Studies On The Location Of Proto-oncogene During Gametogenesis In Cricket, Tekeigryllus Emma

Oncogene is a kind of latent gene in human and other animals , which can cause them into canceration when oncogene is active. In normal cells, it is inactive, so it is called proto-oncogene. Now the study on oncogene is not limited in oncology, and it is involved in cell survival, proliferation, differentiation and information transfer. Many studies indicate that gametogenesis is related to proto-oncogene. Recently, there are many studies on vertebrate and few on invertebrate. Here we select c-kit and c-myc to study the role of proto-oncoogene during gametogenesis in cricket, Tekeigryllus emma (Ohmachi & Matsuura) by immunocytochemistry.The results demonstrate that c-kit and c-myc are expressed in the gametogenesis. The expression of c-kit and c-myc was in specific stages. In oogenesis , c-kit was specifically expressed at high levels in oocytes, and oogonia showed no staining for c-Kit protein; this pattern of expression suggests that c-kit participates in the form of vegetal pole of the oocytes. In spermatogenesis, c-kit was expressed at low levels in spermagonia and spermatocytes, but at high levels in sperm cells, and negative in sperm. These results suggest that c-kit may function in the inhibition of meiotic division of sperm cells. C-myc was expressed at high levels in oocytes; oogonia were negative for Myc protein; these results demostrate that c-myc has regular roles in the oogenesis. In spermatogenesis, there was negative for Myc protein in the spermagonia and sperm; sperm cells showed intense immunostaining for Myc protein, whereas the spermatocytes were no appreciable staining, it is suggested that c-myc plays a role in the second meiotic arrest of sperm cells. This demonstrates that c-kit and c-myc are associated with the process of gametogenesis.The spermatheca is used to stored sperm in female cricket, and the sperm can live 2~3 months in it. In order to find out the reason why insect sperm can live so long, we studied the structure of the spermatheca. The structure ofspermatheca of cricket is observed by light microscope. From inner to outer, it contains inner membrane, gland cells, basal membrane, muscle and peritoneal membrane. These results,on one hand, supply us the foundation to study the factors which affect sperm activity, and on the other hand, play an important role in improving the way of storing sperm.