Research On The Methods For Scientific Visualization Of Multi-Dimensional Images Of Living Cells

Scientific visualization can be used to deal with processing or processed scientific data and to display them on the screen so as to analyze and process these images interactively by image processing and computer graphics technique. Visualization is more and more important as a powerful tool used in scientific research and practice.The purpose of this paper focus on the multi-dimensional deconvolution images used to observe the spatial position and mobility of secretory vesicles of living cell. To meet the needs of scientific visualization, the classical methods and corresponding algorithms are studied, and table a useful proposal for the application of visualization of the multi-dimensional deconvolution images of living cell.This paper consists of four parts. The first part concerns in digital imaging microscope system and three-dimensional fluorescence microscopy that we used in the project, they are the basis of our work. In the second part, we discuss the objects, program and observing methods of scientific visualization. Then two familiar visualization methods, isosurface rendering and direct volume rendering, are studied. Two classical algorithms, marching cubes and ray casting, corresponded to the two methods are demonstrated and used to process the three-dimensional deconvolution images of fluorescence bead and rat pancreatic β cells. The next part of paper concerned in the method and program to research the spatial distribution and mobility of secretory vesicles of living cell, using the combination of four-dimensional fluorescence microscopy and scientific visualization. In the last part, the visualization toolkit is introduced which is the key technique to realize the visualization software VolVision, then discoursed on the structure and functions of VolVision.