Study On The Cryopreservation Of Mouse Embryos

The present research adopted four methods to cryopreserve the mouse morula and early blastocysts. They were programming freezing, quick cooling, vitrification and OPS. Probed into the effect of different Cryo-preservation on the development of mouse embryos, studied from the cryoptectant and seeding temperature etc. In vitro culture and the embryos surgical transfer were used to appraise the effect of mouse embryos cryopreservation.The results showed: 1. The experiments were divided into 3 groups according to three different dosages (5 IU, 10 IU andl5 IU). For the dosage of PMSG treatment, 10IU injection was better than both 5 IU and 10 IU groups(p<0.05).2. The super-ovulated embryos were exposed in four operation solutions as follows:(1) (20%Gly+20%1, 2-Pro) X 30sec.+0.3M Suc. X 1min; (2) (20%EG +20%DMS0) X 30sec. +0.3 M Suc. X 1min; (3) 0.3M Suc. X 1min; (4) Control (no treatment). The survival rates and hatching rates of groups (1) and (2) werelower than that of groups (3) and (4) (P<0.01). 3. Adopted the programming freezing with three cryoprotectant:F1,F2 and F3. percentage of hatching embryos of F1 and F2 were45. 45%, 41. 51%. they both had significantly higher developmental abilities than F3(26. 53%). 4. Mouse embryos were exposed to the cryoprotectant F1 and seeded at -5.5℃ and -6.0℃ followed lauching into liquid nitrogen at -35℃,after thawing and in vitro culture, percentage of hatching embryos were40.38%, 45. 45%. they both had higher developmental...